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Journal of Clinical Microbiology, September 2002, p. 3497-3498, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3497-3498.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Technical Improvement To Prevent DNA Degradation of Enteric Pathogens in Pulsed-Field Gel Electrophoresis

Joanna M. K. Koort,1,{dagger} Susanna Lukinmaa,1 Marjatta Rantala,2 Erja Unkila,3 and Anja Siitonen1*

Laboratory of Enteric Pathogens, National Public Health Institute, Helsinki,1 Kemijärvi City Food Laboratory, Kallaanvaara,2 Savukoski Municipality, Savukoski Finland3

Received 28 January 2002/ Returned for modification 3 April 2002/ Accepted 2 June 2002

This study used a modified pulsed-field gel electrophoresis (PFGE) method with HEPES as a running buffer to prevent electrophoresis-related DNA degradation of nine Salmonella enterica subsp. enterica serovar Ohio, seven Salmonella serovar Newport, and two enterohemorrhagic Escherichia coli (non-O157) strains. All strains yielded identifiable bands with this method in contrast to a commonly applied PFGE method using Tris buffer.

* Corresponding author. Mailing address: National Public Health Institute, Laboratory of Enteric Pathogens, Mannerheimintie 166, FIN-00300 Helsinki, Finland. Phone: 358-9-47448245. Fax: 358-9-47448238. E-mail: anja.siitonen{at}ktl.fi.

{dagger} Present address: Department of Food and Environmental Hygiene, Faculty of Veterinary Medicine, University of Helsinki, Helsinki, Finland.

Journal of Clinical Microbiology, September 2002, p. 3497-3498, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3497-3498.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



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