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Journal of Clinical Microbiology, September 2002, p. 3499-3501, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3499-3501.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.

Early Detection of Negative BACTEC MGIT 960 Cultures by PCR-Reverse Cross-Blot Hybridization Assay

Lucio Romano,1 Maurizio Sanguinetti,1* Brunella Posteraro,1 Fausta Ardito,1 Giovanni Gesu,2 Anna Maria Schito,3 and Giovanni Fadda1

Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Rome,1 Centro Cardiologico "Monzino" IRCCS, Milan,2 Istituto di Microbiologia C. A. Romanzi, Università di Genova, Genoa, Italy3

Received 12 April 2002/ Returned for modification 10 May 2002/ Accepted 15 May 2002

We evaluated the efficacy of a PCR-reverse cross-blot hybridization assay, a test which permits identification of mycobacteria by means of species-specific probes and a Mycobacterium-specific probe, for early detection of negative BACTEC MGIT 960 mycobacterial cultures. Aliquots of 549 cultures were collected 7 days after the culture media were inoculated with various clinical specimens and tested with the molecular assay. PCR results were compared to those obtained at the end times with the BACTEC MGIT 960 system. Of the 549 specimens analyzed, 484 were found to be negative and 64 were found positive by both methods; one specimen, found to be positive by the BACTEC MGIT 960 system, was identified as negative by the molecular assay. In view of its high negative predictive value (99.8%), the PCR-reverse cross-blot hybridization assay appears to be a valid tool for early detection of negative BACTEC MGIT 960 cultures.

* Corresponding author. Mailing address: Istituto di Microbiologia, Università Cattolica del Sacro Cuore, Largo F. Vito, 1, 00168 Rome, Italy. Phone: 39 06 30154964. Fax: 39 06 3051152. E-mail: msanguinetti{at}rm.unicatt.it.

Journal of Clinical Microbiology, September 2002, p. 3499-3501, Vol. 40, No. 9
0095-1137/02/$04.00+0     DOI: 10.1128/JCM.40.9.3499-3501.2002
Copyright © 2002, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Somoskovi, A., Song, Q., Mester, J., Tanner, C., Hale, Y. M., Parsons, L. M., Salfinger, M. (2003). Use of Molecular Methods To Identify the Mycobacterium tuberculosis Complex (MTBC) and Other Mycobacterial Species and To Detect Rifampin Resistance in MTBC Isolates following Growth Detection with the BACTEC MGIT 960 System. J. Clin. Microbiol. 41: 2822-2826 [Abstract] [Full Text]  


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