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Simple and Reliable Method for Detection and Genotyping of Hepatitis C Virus RNA in Dried Blood Spots Stored at Room Temperature

National Institute for Infectious Diseases L. Spallanzani, IRCCS,¹ Central Laboratory Italian Red Cross, Rome, Italy²

Received 13 December 2001/ Returned for modification 14 April 2002/ Accepted 18 May 2002

We describe a simple, sensitive, and reproducible method for using whole blood collected onto filter paper (dried blood spots) for detection and genotyping of hepatitis C virus RNA that can be useful in large field studies, particularly in settings where collection, preparation, storage, and shipment of samples at controlled temperature can be difficult.

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