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Journal of Clinical Microbiology, January 2003, p. 106-109, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.106-109.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Catalase-Negative, Non-Beta-Hemolytic, Gram-Positive Cocci Isolated from Milk Samples

Madeleine Fortin,¹ Serge Messier,^1* Julie Paré,² and Robert Higgins¹

Département de Pathologie et Microbiologie, Faculté de Médecine Vétérinaire, Université de Montréal Saint-Hyacinthe (Québec) J2S 7C6,¹ Laboratoire d'Hygiène Vétérinaire et Alimentaire, Agence Canadienne d’Inspection des Aliments, Saint-Hyacinthe (Québec) J2S 8E3, Canada²

Received 22 April 2002/ Returned for modification 2 September 2002/ Accepted 13 October 2002

This study was undertaken in an effort to improve the identification scheme of catalase-negative, non-beta-hemolytic, gram-positive cocci isolated from milk samples obtained from cows. First, the sensitivity and specificity of the identification procedure currently in use in our laboratory were compared to the results obtained with API 20 STREP strips which were set as the gold standard. Second, a number of other identification tests, which could contribute to increase the sensitivity and specificity of the identification procedure of these microorganisms, were evaluated and selected. The data have shown that there is a necessity to review the identification procedure. Some modifications are suggested to laboratories doing milk sample analyses. A standardized procedure, using the CAMP test, esculin and sodium hippurate hydrolysis, the presence of the enzymes pyrolidonyl arylaminase and leucine aminopeptidase, and acid production from 1% inulin and raffinose broth, would not only improve the results of the identification process of gram-positive cocci isolated from milk samples but also ensure greater uniformity of the epidemiological data.

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* Corresponding author. Mailing address: Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe (Québec) J2S 7C6, Canada. Phone: (450) 773-8521, ext. 8373. Fax: (450) 778-8113. E-mail: serge.messier{at}umontreal.ca.

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Journal of Clinical Microbiology, January 2003, p. 106-109, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.106-109.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

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