This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Landry, M. L. Articles by Ferguson, D. Search for Related Content PubMed PubMed Citation Articles by Landry, M. L. Articles by Ferguson, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, January 2003, p. 346-350, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.346-350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520,¹ Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 06504²

Received 7 June 2002/ Returned for modification 8 August 2002/ Accepted 17 October 2002

Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells.

This article has been cited by other articles:

• Leland, D. S., Ginocchio, C. C. (2007). Role of Cell Culture for Virus Detection in the Age of Technology. Clin. Microbiol. Rev. 20: 49-78 [Abstract] [Full Text]  
• Rutjes, S. A., Italiaander, R., van den Berg, H. H. J. L., Lodder, W. J., de Roda Husman, A. M. (2005). Isolation and Detection of Enterovirus RNA from Large-Volume Water Samples by Using the NucliSens miniMAG System and Real-Time Nucleic Acid Sequence-Based Amplification. Appl. Environ. Microbiol. 71: 3734-3740 [Abstract] [Full Text]  
• Landry, M. L., Garner, R., Ferguson, D. (2005). Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens. J. Clin. Microbiol. 43: 3136-3139 [Abstract] [Full Text]  
• Ginocchio, C. C., Zhang, F., Malhotra, A., Manji, R., Sillekens, P., Foolen, H., Overdyk, M., Peeters, M. (2005). Development, Technical Performance, and Clinical Evaluation of a NucliSens Basic Kit Application for Detection of Enterovirus RNA in Cerebrospinal Fluid. J. Clin. Microbiol. 43: 2616-2623 [Abstract] [Full Text]  
• Landry, M. L., Garner, R., Ferguson, D. (2003). Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens. J. Clin. Microbiol. 41: 5006-5010 [Abstract] [Full Text]  
• Habib-Bein, N. F., Beckwith, W. H. III, Mayo, D., Landry, M. L. (2003). Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus. J. Clin. Microbiol. 41: 3597-3601 [Abstract] [Full Text]