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Journal of Clinical Microbiology, January 2003, p. 346-350, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.346-350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Enterovirus RNA Detection in Clinical Specimens by Using Nucleic Acid Sequence-Based Amplification

Marie L. Landry,1,2* Robin Garner,2 and David Ferguson1,2

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520,1 Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 065042

Received 7 June 2002/ Returned for modification 8 August 2002/ Accepted 17 October 2002

Enterovirus (EV) detection by nucleic acid sequence-based amplification was compared with EV isolation in cell culture. The NucliSens Basic kit (bioMerieux) was utilized for RNA detection. For virus isolation, samples were inoculated into MRC-5, primary rhesus monkey kidney, A549, rhabdomyosarcoma, and/or Buffalo green monkey kidney cells.

* Corresponding author. Mailing address: Department of Laboratory Medicine, P.O. Box 208035, Yale University School of Medicine, New Haven, CT 06520-8035. Phone: (203) 688-3475. Fax: (203) 688-8177. E-mail: marie.landry{at}yale.edu.

Journal of Clinical Microbiology, January 2003, p. 346-350, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.346-350.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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