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Journal of Clinical Microbiology, January 2003, p. 410-413, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.410-413.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Thymidine-Dependent Small-Colony Variants of Staphylococcus aureus Exhibit Gross Morphological and Ultrastructural Changes Consistent with Impaired Cell Separation

Barbara C. Kahl,1* Gunnar Belling,1 Rudolf Reichelt,2 Mathias Herrmann,3 Richard A. Proctor,4 and Georg Peters1

Institute of Medical Microbiology,1 Institute for Medical Physics and Biophysics, University of Muenster Hospital and Clinics, Muenster,2 Institute of Microbiology and Hygiene, University of Saarland, Homburg, Germany,3 Department of Medicine and Medical Microbiology/Immunology, University of Wisconsin Medical School, Madison, Wisconsin4

Received 24 June 2002/ Returned for modification 6 August 2002/ Accepted 8 October 2002

Thymidine-dependent small-colony variants (SCV) of Staphylococcus aureus exhibited unusual colony morphology with "fried-egg" or pinpoint white colonies on agar plates and pleomorphic cocci as determined by Gram staining. Electron microscopy revealed enlarged cocci with incomplete or multiple cross walls consistent with impaired cell separation. Fried-egg SCV phenotypes could be reversed by thymidine supplementation.

* Corresponding author. Mailing address: Inst. f. Med. Mikrobiologie, Domagkstr. 10, 48149 Muenster, Germany. Phone: 49-251-8355381. Fax: 49-251-8355350. E-mail: kahl{at}uni-muenster.de.

Journal of Clinical Microbiology, January 2003, p. 410-413, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.410-413.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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