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Journal of Clinical Microbiology, January 2003, p. 414-420, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.414-420.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of Candida spp. by Randomly Amplified Polymorphic DNA Analysis and Differentiation between Candida albicans and Candida dubliniensis by Direct PCR Methods

Consuelo Bautista-Muñoz, Xavier M. Boldo, Lourdes Villa-Tanaca, and César Hernández-Rodríguez*

Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, I.P.N., Mexico D.F., Mexico

Received 26 February 2002/ Returned for modification 8 May 2002/ Accepted 19 October 2002

Because Candida species have innately highly variable antifungal susceptibilities, the availability of a fast and reliable species identification test is very important so that suitable and effective therapeutic measures may be taken. Using three oligonucleotide primers, we established a randomly amplified polymorphic DNA (RAPD) analysis method that enabled direct identification of the most common opportunistic pathogenic Candida species. RAPD analysis revealed a characteristic molecular fingerprint for each Candida species. Differences between the profiles for Candida albicans and C. dubliniensis were evident. RAPD analysis is a relatively easy, reproducible, and reliable technique that can be useful in providing genetic fingerprints for the identification of strains. In addition, a collection of different C. albicans strains was identified by a specific PCR based on multiple secreted aspartic proteinase (SAP) genes and the dipeptidyl aminopeptidase (DAP2) gene. Our findings demonstrate that PCR based upon the SAP and DAP2 sequences is a simple, rapid, clear, and direct technique for the identification and differentiation of C. albicans and C. dubliniensis.

* Corresponding author. Mailing address: Departamento de Microbiología, Escuela Nacional de Ciencias Biológicas, I.P.N., Apartado Postal CON 174, México, D.F. CP 06400, Mexico. Phone and fax: (52 55) 57 29 62 09. E-mail: chdez38{at}hotmail.com.

Journal of Clinical Microbiology, January 2003, p. 414-420, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.414-420.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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