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Journal of Clinical Microbiology, January 2003, p. 428-431, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.428-431.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Phenotypes and Genotypes of Erythromycin-Resistant Pneumococci in Italy

Maria Pia Montanari, Marina Mingoia, Ileana Cochetti, and Pietro Emanuele Varaldo*

Department of Microbiology and Biomedical Sciences, University of Ancona Medical School, 60131 Ancona, Italy

Received 5 August 2002/ Returned for modification 23 September 2002/ Accepted 6 October 2002

Of 120 erythromycin-resistant pneumococci isolated in Italian hospitals, 39 (32.5%) were M-type isolates, carrying the mef gene alone. The mef gene was also detected, together with erm(AM), in one constitutively resistant isolate and in five isolates of the partially inducible phenotype. Among the 45 mef-positive isolates, 25 (55.6%) carried mef(A) and 20 (44.4%) carried mef(E) as observed from PCR-restriction fragment length polymorphism analysis of a 1,743-bp amplicon. The same result was obtained by a similar method applied to a more common 348-bp amplicon.

* Corresponding author. Mailing address: Department of Microbiology and Biomedical Sciences, University of Ancona Medical School, Via Ranieri, Monte d'Ago, 60131 Ancona, Italy. Phone: 39 071 2204694. Fax: 39 071 2204693. E-mail: pe.varaldo{at}unian.it.

Journal of Clinical Microbiology, January 2003, p. 428-431, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.428-431.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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