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Journal of Clinical Microbiology, January 2003, p. 432-434, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.432-434.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Antigen Detection Assay for Identification of Penicillium marneffei Infection

Sansanee C. Chaiyaroj,1,2* Runglawan Chawengkirttikul,1 Stitaya Sirisinha,1,2 Pramuan Watkins,1 and Yuttana Srinoulprasert1

Department of Microbiology, Faculty of Science, Mahidol University,1 Laboratory of Immunology, Chulabhorn Research Institute, Bangkok, Thailand2

Received 8 April 2002/ Returned for modification 29 September 2002/ Accepted 31 October 2002

Two recently produced monoclonal antibodies were used to develop an antigen capture enzyme-linked immunosorbent assay (ELISA) for rapid diagnosis of Penicillium marneffei. The method was evaluated with 53 patients with culture-confirmed penicilliosis and 240 controls. The diagnostic sensitivity, specificity, and accuracy of the ELISA were 92.45, 97.5, and 96.59%, respectively.

* Corresponding author. Mailing address: Department of Microbiology, Faculty of Science, Mahidol University, Bangkok 10400, Thailand. Phone: 662 246 1358. Fax: 662 644 5411. E-mail: scscy{at}mahidol.ac.th.

Journal of Clinical Microbiology, January 2003, p. 432-434, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.432-434.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Huang, Y.-T., Hung, C.-C., Liao, C.-H., Sun, H.-Y., Chang, S.-C., Chen, Y.-C. (2007). Detection of Circulating Galactomannan in Serum Samples for Diagnosis of Penicillium marneffei Infection and Cryptococcosis among Patients Infected with Human Immunodeficiency Virus. J. Clin. Microbiol. 45: 2858-2862 [Abstract] [Full Text]  


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