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Journal of Clinical Microbiology, January 2003, p. 442-446, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.442-446.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Sequence Polymorphism of the 16S rRNA Gene of Vibrio vulnificus Is a Possible Indicator of Strain Virulence

William B. Nilsson,1 Rohinee N. Paranjype,1 Angelo DePaola,2 and Mark S. Strom1*

Northwest Fisheries Science Center, National Marine Fisheries Service, National Oceanic and Atmospheric Administration, U.S. Department of Commerce, Seattle, Washington 98112,1 Gulf Coast Seafood Laboratory, U.S. Food and Drug Administration, Dauphin Island, Alabama 365282

Received 31 July 2002/ Returned for modification 14 September 2002/ Accepted 23 October 2002

Vibrio vulnificus exhibits considerable strain-to-strain variation in virulence. Attempts to associate phenotypic or genotypic characteristics with strain virulence have been largely unsuccessful. Based on a 17-nucleotide difference throughout the sequence of the small subunit 16S rRNA gene, there are two major groups of V. vulnificus designated types A and B. In a survey of the 16S rRNA genotype in 67 V. vulnificus human clinical and nonclinical strains, we determined that the majority of nonclinical isolates are type A (31 of 33) and that there is a statistically significant association between the type B genotype and human clinical strains (26 of 34).

* Corresponding author. Mailing address: 2725 Montlake Blvd. E., Seattle, WA 98112. Phone: (206) 860-3377. Fax: (206) 860-3467. E-mail: mark.strom{at}noaa.gov.

Journal of Clinical Microbiology, January 2003, p. 442-446, Vol. 41, No. 1
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.1.442-446.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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