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Journal of Clinical Microbiology, October 2003, p. 4589-4593, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4589-4593.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Protocol Using Gene Capture and PCR for Detection of Helicobacter pylori DNA in Feces

W. G. MacKay,1* C. L. Williams,1 M. McMillan,1 R. N. Ndip,2 A. J. Shepherd,3 and L. T. Weaver1

Division of Developmental Medicine, University of Glasgow, Glasgow,1 Department of Nursing and Midwifery, University of Stirling, Stirling, Scotland,3 Department of Life Sciences, University of Buea, Buea, Cameroon2

Received 8 January 2003/ Returned for modification 1 February 2003/ Accepted 24 June 2003

The route of transmission of Helicobacter pylori, which is usually acquired in childhood and is one of the most common bacterial infections in humans, remains undetermined. Mapping the distribution of H. pylori genotypes within families could help to determine the routes of transmission and risk factors. Here we describe a noninvasive method for obtaining H. pylori DNA isolates from the feces of children. Children presenting with gastrointestinal symptoms at the Royal Hospital for Sick Children were tested for gastric H. pylori colonization by using the 13C-urea breath test (UBT) and were asked to provide fecal samples, which were tested for H. pylori by using the HpSA fecal antigen test. DNA was purified from fecal samples by using a novel method of gene capture with subsequent H. pylori PCR analysis. Fifteen UBT-positive and 15 UBT-negative children participated in the study. The positive and negative predictive values for the assay were 80 and 100%, respectively. Fecal DNA purification followed by H. pylori PCR analysis is an effective tool for harvesting H. pylori DNA isolates from the feces of children. This technique may be developed to allow the diagnosis and noninvasive genotyping of H. pylori in children and their families.

* Corresponding author. Mailing address: Division of Developmental Medicine, University of Glasgow, Yorkhill Hospitals, Glasgow G3 8SJ, Scotland. Phone: 44 (0)141 201 0867. Fax: 44 (0)141 201 0837. E-mail: wgmk1d{at}clinmed.gla.ac.uk.

Journal of Clinical Microbiology, October 2003, p. 4589-4593, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4589-4593.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Megraud, F., Lehours, P. (2007). Helicobacter pylori Detection and Antimicrobial Susceptibility Testing. Clin. Microbiol. Rev. 20: 280-322 [Abstract] [Full Text]  
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