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Journal of Clinical Microbiology, October 2003, p. 4783-4786, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4783-4786.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid and Specific Detection of Mycobacterium tuberculosis by Using the Smart Cycler Instrument and a Specific Fluorogenic Probe

Timothy J. Cleary,1,2* Gladys Roudel,1 Ofelia Casillas,1 and Nancimae Miller1,{dagger}

Department of Pathology,1 Department of Microbiology and Immunology, University of Miami/Jackson Memorial Medical Center, Miami, Florida 331362

Received 19 February 2003/ Returned for modification 31 March 2003/ Accepted 22 July 2003

A procedure using the Smart Cycler instrument and a fluorescence quencher (FQ) probe for the specific identification of Mycobacterium tuberculosis complex (MTB) was used to detect organisms in 366 acid-fast bacillus smear-positive respiratory specimens. It was compared to culture and the AMPLICOR M. tuberculosis PCR test. MTB was isolated from 198 of these samples. The FQ PCR assay was sensitive (197 of 198, 99.5%) and specific (165 of 168, 98.2%); no significant difference was observed between the two PCR protocols. After DNA extraction, a final result was available within 1.5 h with the real-time PCR protocol.

* Corresponding author. Mailing address: Department of Pathology (D33), 1611 NW 12 Ave., Holtz Tower 2092, Miami, FL 33136. Phone: (305) 585-7851. Fax: (305) 585-0008. E-mail: tcleary{at}med.miami.edu.

{dagger} Present address: Department of Pathology, Mt. Sinai Medical Center, Miami Beach, Florida.

Journal of Clinical Microbiology, October 2003, p. 4783-4786, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4783-4786.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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