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Journal of Clinical Microbiology, October 2003, p. 4812-4814, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4812-4814.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Cellular Fatty Acid Profile Distinguishes Burkholderia pseudomallei from Avirulent Burkholderia thailandensis

Division of Microbiology and Infectious Diseases,¹ Division of Clinical Pathology, Western Australian Centre for Pathology and Medical Research,² Microbiology, School of Biomedical and Chemical Sciences, University of Western Australia, Nedlands,⁵ Department of Medicine, University of Western Australia, Perth, Western Australia, Australia,³ Wellcome Tropical Diseases Research Unit, Mahidol University, Bangkok, Thailand⁴

Received 4 June 2003/ Returned for modification 7 July 2003/ Accepted 14 July 2003

Burkholderia pseudomallei, the cause of melioidosis, can be distinguished from the closely related but nonpathogenic Burkholderia thailandensis by gas chromatography (GC) analysis of fatty acid derivatives. A 2-hydroxymyristic acid derivative (14:0 2OH) was present in 95% of B. pseudomallei isolates and no B. thailandensis isolates. GC mass spectrophotometry confirmed that 2-hydroxymyristic acid was present in B. pseudomallei. GC-fatty acid methyl ester analysis may be useful in distinguishing these two closely related species.

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