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Journal of Clinical Microbiology, October 2003, p. 4815-4816, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4815-4816.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Real-Time PCR Assay Targets the 23S-5S Spacer for Direct Detection and Differentiation of Legionella spp. and Legionella pneumophila

Bjorn L. Herpers,1* Bartelt M. de Jongh,1 Kim van der Zwaluw,2 and Erik J. van Hannen1

Department of Medical Microbiology and Immunology, St. Antonius Hospital, Koekoekslaan 1, 3435 CM Nieuwegein,1 Diagnostic Laboratory for Infectious Diseases and Perinatal Screening (LIS), National Institute of Public Health and the Environment (RIVM), 3720 BA Bilthoven, The Netherlands2

Received 15 May 2003/ Returned for modification 19 June 2003/ Accepted 14 July 2003

A real-time PCR for the ABI Prism 7000 system targeting the 23S-5S spacer of Legionella spp. was developed. Simultaneous detection and differentiation of Legionella spp. and Legionella pneumophila within 90 min and without post-PCR melting-curve analysis was achieved using two TaqMan probes. In sputum samples from 23 controls and 17 patients with legionellosis, defined by positive culture, urinary antigen testing, or seroconversion, 94% sensitivity and 100% specificity were observed.

* Corresponding author. Mailing address: Department of Medical Microbiology and Immunology, St. Antonius Hospital, P.O. Box 2500, 3430 EM Nieuwegein, The Netherlands. Phone: 31 (0)30-6093032. Fax: 31 (0)30-6092429. E-mail: b.herpers{at}antonius.net.

Journal of Clinical Microbiology, October 2003, p. 4815-4816, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4815-4816.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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