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Journal of Clinical Microbiology, October 2003, p. 4820-4822, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4820-4822.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Amplified-Fragment Length Polymorphism as a Complement to IS6110-Based Restriction Fragment Length Polymorphism Analysis for Molecular Typing of Mycobacterium tuberculosis

Monserrat Ruiz,1* Juan Carlos Rodríguez,1 Francisco Rodríguez-Valera,2 and Gloria Royo1,2

Section of Microbiology, Hospital General Universitario de Elche,1 Department of Microbiology, Universidad Miguel Hernández, Elche (Alicante), Spain2

Received 5 March 2003/ Returned for modification 31 March 2003/ Accepted 10 June 2003

The amplified-fragment length polymorphism (AFLP) technique was applied to clusters of Mycobacterium tuberculosis clinical isolates obtained by using IS6110-based restriction fragment length polymorphism (RFLP). Ten of the RFLP clusters showed identical AFLP patterns also, but the other 13 could be resolved into subclusters by AFLP. Our results suggest that some RFLP clusters may not be due to recent transmission and that AFLP may be a useful complementary technique.

* S. Microbiología, Hospital General Universitario de Elche, Camí de L'Almazara, no. 11, 03203 Elche (Alicante), Spain. Phone: 34-966679048. Fax: 34-966679108. E-mail: micro_elx{at}gva.es.

Journal of Clinical Microbiology, October 2003, p. 4820-4822, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4820-4822.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Kassama, Y., Shemko, M., Shetty, N., Fang, Z., MacIntire, G., Gant, V., Zumla, A., Goodacre, R. (2006). An Improved Fluorescent Amplified Fragment Length Polymorphism Method for Typing Mycobacterium tuberculosis. J. Clin. Microbiol. 44: 288-289 [Full Text]  


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