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Journal of Clinical Microbiology, October 2003, p. 4826-4828, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4826-4828.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Diagnosis of Congenital Toxoplasmosis

Comparison of Targets for Detection of Toxoplasma gondii by PCR

Denis Filisetti,* Mohamed Gorcii, Elodie Pernot-Marino, Odile Villard, and Ermanno Candolfi

Institut de Parasitologie et de Pathologie Tropicale, Strasbourg, France

Received 13 March 2003/ Returned for modification 4 May 2003/ Accepted 13 July 2003

Three PCR targets (18S ribosomal DNA, B1, and AF146527) and mouse inoculation were compared for 83 samples in the context of congenital toxoplasmosis. These four techniques are not statistically different in terms of sensitivity and specificity. However, further analysis highlighted problems sometimes encountered with PCR diagnosis of congenital toxoplasmosis.

* Corresponding author. Mailing address: Institut de Parasitologie et de Pathologie Tropicale, 3 rue Koeberlé, 67000 Strasbourg, France. Phone: 33 390 24 37 00. Fax: 33 390 24 36 93. E-mail: denis.filisetti{at}medecine.u-strasbg.fr.

Journal of Clinical Microbiology, October 2003, p. 4826-4828, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4826-4828.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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