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Journal of Clinical Microbiology, October 2003, p. 4844-4847, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4844-4847.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Differentiation of Mycoplasma Species by 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis Fingerprinting

Laura McAuliffe,1* Richard J. Ellis,2 Roger D. Ayling,1 and Robin A. J. Nicholas1

Mycoplasma Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Surrey, KT15 3NB,1 NERC Centre for Population Biology, Imperial College London, Silwood Park Campus, Ascot, Berks, SL5 7PY, United Kingdom2

Received 18 February 2003/ Returned for modification 16 April 2003/ Accepted 27 July 2003

Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and serological tests.

* Corresponding author. Mailing address: Mycoplasma Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Woodham Ln., Addlestone, Surrey, United Kingdom KT15 3NB. Phone: (44) 1932 357918. Fax: (44) 1932 357423. E-mail: l.mcauliffe{at}vla.defra.gsi.gov.uk.

Journal of Clinical Microbiology, October 2003, p. 4844-4847, Vol. 41, No. 10
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.10.4844-4847.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Stakenborg, T., Vicca, J., Verhelst, R., Butaye, P., Maes, D., Naessens, A., Claeys, G., De Ganck, C., Haesebrouck, F., Vaneechoutte, M. (2005). Evaluation of tRNA Gene PCR for Identification of Mollicutes. J. Clin. Microbiol. 43: 4558-4566 [Abstract] [Full Text]  
  • McAuliffe, L., Ellis, R. J, Lawes, J. R, Ayling, R. D, Nicholas, R. A. (2005). 16S rDNA PCR and denaturing gradient gel electrophoresis; a single generic test for detecting and differentiating Mycoplasma species. J Med Microbiol 54: 731-739 [Abstract] [Full Text]  


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