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Differentiation of Mycoplasma Species by 16S Ribosomal DNA PCR and Denaturing Gradient Gel Electrophoresis Fingerprinting

Mycoplasma Group, Department of Bacterial Diseases, Veterinary Laboratories Agency (Weybridge), Surrey, KT15 3NB,¹ NERC Centre for Population Biology, Imperial College London, Silwood Park Campus, Ascot, Berks, SL5 7PY, United Kingdom²

Received 18 February 2003/ Returned for modification 16 April 2003/ Accepted 27 July 2003

Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species of veterinary significance. Twenty-seven (85%) species could be differentiated by DGGE. This method could enable the rapid identification of many mycoplasma species for which there is no specific PCR available and which are currently identified by using culture and serological tests.

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