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Journal of Clinical Microbiology, November 2003, p. 4930-4940, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.4930-4940.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Association of Genomic O Island 122 of Escherichia coli EDL 933 with Verocytotoxin-Producing Escherichia coli Seropathotypes That Are Linked to Epidemic and/or Serious Disease

Mohamed A. Karmali,1,2* Mariola Mascarenhas,1 Songhai Shen,1 Kim Ziebell,1 Shelley Johnson,1 Richard Reid-Smith,1 Judith Isaac-Renton,3,4 Clifford Clark,5 Kris Rahn,1 and James B. Kaper6

Laboratory for Foodborne Zoonoses, Population and Public Health Branch, Health Canada, Guelph,1 Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ontario,2 British Columbia Centre for Disease Control,3 Department of Pathology and Laboratory Medicine, University of British Columbia, Vancouver, British Columbia,4 National Microbiology Laboratory, Population and Public Health Branch, Health Canada, Winnipeg, Manitoba, Canada,5 Center for Vaccine Development, University of Maryland School of Medicine, Baltimore, Maryland6

Received 21 May 2003/ Returned for modification 22 July 2003/ Accepted 20 August 2003

The distribution of EDL 933 O island 122 (OI-122) was investigated in 70 strains of Verocytotoxin-producing Escherichia coli (VTEC) of multiple serotypes that were classified into five "seropathotypes" (A through E) based on the reported occurrence of serotypes in human disease, in outbreaks, and/or in the hemolytic-uremic syndrome (HUS). Seropathotype A comprised 10 serotype O157:H7 and 3 serotype O157:NM strains. Seropathotype B (associated with outbreaks and HUS but less commonly than serotype O157:H7) comprised three strains each of serotypes O26:H11, O103:H2, O111:NM, O121:H19, and O145:NM. Seropathotype C comprised four strains each of serotypes O91:H21 and O113:H21 and eight strains of other serotypes that have been associated with sporadic HUS but not typically with outbreaks. Seropathotype D comprised 14 strains of serotypes that have been associated with diarrhea but not with outbreaks or HUS, and seropathotype E comprised animal VTEC strains of serotypes not implicated in human disease. All strains were tested for four EDL 933 OI-122 virulence genes (Z4321, Z4326, Z4332, and Z4333) by PCR. Negative PCRs were confirmed by Southern hybridization. Overall, 28 (40%) strains contained OI-122 (positive for all four virulence genes), 27 (38.6%) contained an "incomplete" OI-122 (positive for one to three genes), and 15 (21.4%) strains did not contain OI-122. The seropathotype distribution of complete OI-122 was as follows: 100% for seropathotype A, 60% for B, 36% for C, 15% for D, and 0% for E. The differences in the frequency of OI-122 between seropathotypes A, B, and C (associated with HUS) and seropathotypes D and E (not associated with HUS) and between seropathotypes A and B (associated with epidemic disease) and seropathotypes C, D, and E (not associated with epidemic disease) were highly significant (P < 0.0001).

* Corresponding author. Mailing address: Laboratory for Foodborne Zoonoses, Population and Public Health Branch, Health Canada, 110 Stone Rd. West, Guelph, Ontario, Canada N1G 3W4. Phone: (519) 822-3300. Fax: (519) 822-2280. E-mail: Mohamed_Karmali{at}hc-sc.gc.ca.

Journal of Clinical Microbiology, November 2003, p. 4930-4940, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.4930-4940.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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