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Journal of Clinical Microbiology, November 2003, p. 5006-5010, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5006-5010.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens

Marie L. Landry,1,2* Robin Garner,2 and David Ferguson1,2

Department of Laboratory Medicine, Yale University School of Medicine, New Haven, Connecticut 06520,1 Clinical Virology Laboratory, Yale New Haven Hospital, New Haven, Connecticut 065042

Received 14 March 2003/ Returned for modification 19 July 2003/ Accepted 18 August 2003

Samples were tested for enterovirus by nucleic acid sequence-based amplification (NASBA) (NucliSens Basic kit; BioMerieux), reverse transcription-PCR (RT-PCR) (Enterovirus Consensus RT-PCR kit; Argene Biosoft), and virus isolation. Eighty-two samples were tested, and 44 were positive, 34 by both NASBA and RT-PCR and 5 each by NASBA or RT-PCR only. Two nasopharyngeal samples positive only by RT-PCR were determined to be rhinovirus. Of 42 enterovirus-positive samples, NASBA detected 39 (92.9%) and RT-PCR detected 37 (88.1%). The NucliSens Basic kit and the Argene Biosoft RT-PCR had comparable sensitivities for detection of enterovirus RNA, and both molecular methods were more sensitive than culture, which detected only 60.5% of positive samples. NASBA could be completed in 6.5 h versus 9 h for the Argene Biosoft RT-PCR kit.

* Corresponding author. Mailing address: Department of Laboratory Medicine, P.O. Box 208035, Yale University School of Medicine, New Haven, CT 06520-8035. Phone: (203) 688-3475. Fax: (203) 688-8177. E-mail: marie.landry{at}yale.edu.

Journal of Clinical Microbiology, November 2003, p. 5006-5010, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5006-5010.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Mahony, J. B. (2008). Detection of Respiratory Viruses by Molecular Methods. Clin. Microbiol. Rev. 21: 716-747 [Abstract] [Full Text]  
  • Landry, M. L., Garner, R., Ferguson, D. (2005). Real-Time Nucleic Acid Sequence-Based Amplification Using Molecular Beacons for Detection of Enterovirus RNA in Clinical Specimens. J. Clin. Microbiol. 43: 3136-3139 [Abstract] [Full Text]  
  • Ginocchio, C. C., Zhang, F., Malhotra, A., Manji, R., Sillekens, P., Foolen, H., Overdyk, M., Peeters, M. (2005). Development, Technical Performance, and Clinical Evaluation of a NucliSens Basic Kit Application for Detection of Enterovirus RNA in Cerebrospinal Fluid. J. Clin. Microbiol. 43: 2616-2623 [Abstract] [Full Text]  


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