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Journal of Clinical Microbiology, November 2003, p. 5041-5045, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5041-5045.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection and Identification of Entamoeba Species in Stool Samples by a Reverse Line Hybridization Assay

Jaco J. Verweij,* Daphne Laeijendecker, Eric A. T. Brienen, Lisette van Lieshout, and Anton M. Polderman

Department of Parasitology, Leiden University Medical Centre, Leiden, The Netherlands

Received 28 May 2003/ Returned for modification 16 July 2003/ Accepted 14 August 2003

Classically, detection of Entamoeba histolytica is performed by microscopic examination for characteristic cysts and/or trophozoites in fecal preparations. Differentiation of E. histolytica cysts and those of nonpathogenic amoebic species is made on the basis of the appearance and the size of the cysts. However, by classical means objective tools for confirmation and quality control do not exist. Therefore, a reverse line blot hybridization assay was developed to detect a variety of Entamoeba species and genetic variants known to infect humans. The assay was performed after amplification with general Entamoeba-specific primers. The assay could identify four genetic variants of Entamoeba polecki-like cysts as well as E. histolytica, Entamoeba dispar, Entamoeba hartmanni, Entamoeba moshkovskii and Entamoeba coli and even mixed infections in a range of controls and fecal samples. This technique can be used as an additional standard for diagnosis, epidemiology, and quality control for amoebic infections.

* Corresponding author. Mailing address: Department of Parasitology, Leiden University Medical Centre, P.O. Box 9600, 2300 RC Leiden, The Netherlands. Phone: 31-71-5265080. Fax: 31-71-5266907. E-mail: j.j.verweij{at}lumc.nl.

Journal of Clinical Microbiology, November 2003, p. 5041-5045, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5041-5045.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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