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Journal of Clinical Microbiology, November 2003, p. 5059-5065, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5059-5065.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Genetic Diversity among Borrelia Strains Determined by Single-Strand Conformation Polymorphism Analysis of the ospC Gene and Its Association with Invasiveness

Vanessa Lagal,1* Danièle Postic,1 Eva Ruzic-Sabljic,2 and Guy Baranton1

Unité de Bactériologie Moléculaire et Médicale, Institut Pasteur, 75724 Cedex 15, France,1 Institute of Microbiology and Immunology, 1000 Ljubljana, Slovenia2

Received 30 October 2002/ Returned for modification 5 March 2003/ Accepted 8 June 2003

Lyme borreliosis (LB) is a tick-borne spirochetal infection caused by three Borrelia species: Borrelia afzelii, B. garinii, and B. burgdorferi sensu stricto. LB evolves in two stages: a skin lesion called erythema migrans and later, different disseminated forms (articular, neurological, cardiac...). Previous research based on analysis of ospC sequences allowed the definition of 58 groups (divergence of <2% within a group and >8% between groups). Only 10 of these groups include all of the strains isolated from disseminated forms that are considered invasive. The aim of this study was to determine whether or not invasive strains belong to restricted ospC groups by testing human clinical strains isolated from disseminated forms. To screen for ospC genetic diversity, we used single-strand conformation polymorphism (SSCP) analysis. Previously known ospC sequences from 44 different strains were first tested, revealing that each ospC group had a characteristic SSCP pattern. Therefore, we studied 80 disseminated-form isolates whose ospC sequences were unknown. Of these, 28 (35%) belonged to previously known invasive groups. Moreover, new invasive groups were identified: six of B. afzelii, seven of B. garinii, and one of B. burgdorferi sensu stricto. This study confirmed that invasive strains are not distributed among all 69 ospC groups but belong to only 24 groups. This suggests that OspC may be involved in the invasiveness of B. burgdorferi.

* Corresponding author. Mailing address: Institut Pasteur, Unité de Bactériologie Moléculaire et Médicale, 28, rue du Dr Roux, 75724 Paris Cedex 15, France. Phone: 33 1 45 68 83 37. Fax: 33 1 40 61 30 01. E-mail: vlagal{at}pasteur.fr.

Journal of Clinical Microbiology, November 2003, p. 5059-5065, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5059-5065.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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