This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hall, L. Articles by Roberts, G. D. Search for Related Content PubMed PubMed Citation Articles by Hall, L. Articles by Roberts, G. D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2003, p. 5099-5102, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5099-5102.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Commonly Encountered, Clinically Important Yeast Species

Division of Clinical Microbiology, Mayo Clinic, Rochester, Minnesota 55905

Received 8 April 2003/ Returned for modification 24 June 2003/ Accepted 4 August 2003

Experience with a MicroSeq D2 large-subunit (LSU) ribosomal DNA (rDNA) sequencing kit for identification of yeast species commonly encountered in the mycology laboratory at Mayo Clinic is described here. A total of 131 isolates of yeasts recovered from clinical specimens were included in the study. Phenotypic methods used for initial identification included germ tube formation, urease production, microscopic morphological features on cornmeal agar, and an API 20C AUX system; all isolates were sequenced using a MicroSeq D2 LSU rDNA sequencing kit. Nucleic acid sequencing identified 93.9% of the isolates to the correct genus and species. A total of 100 of the isolates (representing 19 species of Candida) were sequenced, and 98% gave results concordant with identifications made by the API 20C AUX system; distance scores ranged from 0 to 1.88%, with an average value of 0.23%. Candida dubliniensis was not included in the MicroSeq database and was identified as Candida albicans. A total of 32 isolates representing 9 other genera (including Cryptococcus, Filobasidium, Kloeckera, Malassezia, Pichia, Sporidiobolus, Rhodotorula, Zygosaccharomyces, and Trichosporon) were included, and 81.3% showed concordant results when phenotypic and sequencing results were compared. Most discrepancies were attributed to the lack of inclusion of the species in the MicroSeq or API 20C AUX database. The MicroSeq D2 LSU rDNA sequencing kit appears to be accurate and useful for the identification of yeasts that might be seen in a clinical laboratory.

This article has been cited by other articles:

• Lockhart, S. R., Messer, S. A., Pfaller, M. A., Diekema, D. J. (2008). Lodderomyces elongisporus Masquerading as Candida parapsilosis as a Cause of Bloodstream Infections. J. Clin. Microbiol. 46: 374-376 [Abstract] [Full Text]  
• Hata, D. J., Hall, L., Fothergill, A. W., Larone, D. H., Wengenack, N. L. (2007). Multicenter Evaluation of the New VITEK 2 Advanced Colorimetric Yeast Identification Card. J. Clin. Microbiol. 45: 1087-1092 [Abstract] [Full Text]  
• Sanguinetti, M., Porta, R., Sali, M., La Sorda, M., Pecorini, G., Fadda, G., Posteraro, B. (2007). Evaluation of VITEK 2 and RapID Yeast Plus Systems for Yeast Species Identification: Experience at a Large Clinical Microbiology Laboratory. J. Clin. Microbiol. 45: 1343-1346 [Abstract] [Full Text]  
• Page, B. T., Shields, C. E., Merz, W. G., Kurtzman, C. P. (2006). Rapid identification of ascomycetous yeasts from clinical specimens by a molecular method based on flow cytometry and comparison with identifications from phenotypic assays.. J. Clin. Microbiol. 44: 3167-3171 [Abstract] [Full Text]  
• Rakeman, J. L., Bui, U., LaFe, K., Chen, Y.-C., Honeycutt, R. J., Cookson, B. T. (2005). Multilocus DNA Sequence Comparisons Rapidly Identify Pathogenic Molds. J. Clin. Microbiol. 43: 3324-3333 [Abstract] [Full Text]  
• Wilson, D. A., Joyce, M. J., Hall, L. S., Reller, L. B., Roberts, G. D., Hall, G. S., Alexander, B. D., Procop, G. W. (2005). Multicenter Evaluation of a Candida albicans Peptide Nucleic Acid Fluorescent In Situ Hybridization Probe for Characterization of Yeast Isolates from Blood Cultures. J. Clin. Microbiol. 43: 2909-2912 [Abstract] [Full Text]  
• Hinrikson, H. P., Hurst, S. F., Lott, T. J., Warnock, D. W., Morrison, C. J. (2005). Assessment of Ribosomal Large-Subunit D1-D2, Internal Transcribed Spacer 1, and Internal Transcribed Spacer 2 Regions as Targets for Molecular Identification of Medically Important Aspergillus Species. J. Clin. Microbiol. 43: 2092-2103 [Abstract] [Full Text]  
• de Aguirre, L., Hurst, S. F., Choi, J. S., Shin, J. H., Hinrikson, H. P., Morrison, C. J. (2004). Rapid Differentiation of Aspergillus Species from Other Medically Important Opportunistic Molds and Yeasts by PCR-Enzyme Immunoassay. J. Clin. Microbiol. 42: 3495-3504 [Abstract] [Full Text]  
• Hall, L., Wohlfiel, S., Roberts, G. D. (2004). Experience with the MicroSeq D2 Large-Subunit Ribosomal DNA Sequencing Kit for Identification of Filamentous Fungi Encountered in the Clinical Laboratory. J. Clin. Microbiol. 42: 622-626 [Abstract] [Full Text]