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Journal of Clinical Microbiology, November 2003, p. 5202-5206, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5202-5206.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Variation in the Variable-Length PCR Target and 120-Kilodalton Antigen Genes of Ehrlichia chaffeensis from White-Tailed Deer (Odocoileus virginianus)

Michael J. Yabsley,1,2* Susan E. Little,2 Ethan J. Sims,2 Vivien G. Dugan,1,2 David E. Stallknecht,1,2 and William R. Davidson1,3

Southeastern Cooperative Wildlife Disease Study,1 Department of Medical Microbiology and Parasitology, College of Veterinary Medicine,2 D. B. Warnell School of Forest Resources, The University of Georgia, Athens, Georgia3

Received 28 May 2003/ Returned for modification 10 July 2003/ Accepted 20 August 2003

Genes encoding two surface-expressed antigens of Ehrlichia chaffeensis, the variable-length PCR target (VLPT) and the 120-kDa antigen, which contain variable numbers of tandem repeats, were characterized for E. chaffeensis from white-tailed deer (Odocoileus virginianus). Both genes from infected deer contained numbers of repeats similar to those reported in genes from humans and ticks, although a new variant of the 120-kDa antigen gene containing five repeat units and coinfection with multiple VLPT and 120-kDa antigen gene genetic types were detected. Sequence analysis of both genes revealed more nucleotide variation than previously reported for E. chaffeensis from infected humans or ticks. This is the most extensive study of E. chaffeensis VLPT and 120-kDa antigen gene genetic variation to date and is the first to examine genetic variation in E. chaffeensis from a nonhuman vertebrate host.

* Corresponding author. Mailing address: Wildlife Health Building, Southeastern Cooperative Wildlife Disease Study, College of Veterinary Medicine, University of Georgia, Athens, GA 30602. Phone: (706) 542-1741. Fax: (706) 542-5865. E-mail: myabsley{at}vet.uga.edu.

Journal of Clinical Microbiology, November 2003, p. 5202-5206, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5202-5206.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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