This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrowReprints and Permissions
Right arrow Copyright Information
Right arrow Books from ASM Press
Right arrow MicrobeWorld
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Rodriguez-Tudela, J. L.
Right arrow Articles by Cuenca-Estrella, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Rodriguez-Tudela, J. L.
Right arrow Articles by Cuenca-Estrella, M.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2003, p. 5236-5237, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5236-5237.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Interlaboratory Evaluation of Hematocytometer Method of Inoculum Preparation for Testing Antifungal Susceptibilities of Filamentous Fungi

J. L. Rodriguez-Tudela,1 Erja Chryssanthou,2 Evangelia Petrikkou,1 Juan Mosquera,3 David W. Denning,3 and Manuel Cuenca-Estrella1*

Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Spain,1 Karolinska Hospital, Stockholm, Sweden,2 University of Manchester, Manchester, United Kingdom3

Received 22 July 2003/ Returned for modification 5 June 2003/ Accepted 4 August 2003

A three-laboratory study was performed to evaluate conidium counting in a hematocytometer as a technique of inoculum preparation for susceptibility testing of Aspergillus spp. In addition, inocula were quantified by colony counting and optical density determination. The agreement and correlation coefficient between conidium and colony quantifications were 89.2% and 0.73 (P < 0.01). Correlations with optical density determination were not significant.

* Corresponding author. Mailing address: Unidad de Micología, Centro Nacional de Microbiología, Instituto de Salud Carlos III, Ctra Majadahonda-Pozuelo Km 2, 28220 Majadahonda (Madrid), Spain. Phone: 34-91-5097961. Fax: 34-91-5097966. E-mail: mcuenca-estrella{at}isciii.es.

Journal of Clinical Microbiology, November 2003, p. 5236-5237, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5236-5237.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Alastruey-Izquierdo, A., Castelli, M. V., Cuesta, I., Monzon, A., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2009). Activity of Posaconazole and Other Antifungal Agents against Mucorales Strains Identified by Sequencing of Internal Transcribed Spacers. Antimicrob. Agents Chemother. 53: 1686-1689 [Abstract] [Full Text]  
  • Tezcan, G., Ozhak-Baysan, B., Alastruey-Izquierdo, A., Ogunc, D., Ongut, G., Yildiran, S. T., Hazar, V., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2009). Disseminated Fusariosis Caused by Fusarium verticillioides in an Acute Lymphoblastic Leukemia Patient after Allogeneic Hematopoietic Stem Cell Transplantation. J. Clin. Microbiol. 47: 278-281 [Abstract] [Full Text]  
  • Castelli, M. V., Alastruey-Izquierdo, A., Cuesta, I., Monzon, A., Mellado, E., Rodriguez-Tudela, J. L., Cuenca-Estrella, M. (2008). Susceptibility Testing and Molecular Classification of Paecilomyces spp.. Antimicrob. Agents Chemother. 52: 2926-2928 [Abstract] [Full Text]  
  • Rodriguez-Tudela, J. L., Alcazar-Fuoli, L., Mellado, E., Alastruey-Izquierdo, A., Monzon, A., Cuenca-Estrella, M. (2008). Epidemiological Cutoffs and Cross-Resistance to Azole Drugs in Aspergillus fumigatus. Antimicrob. Agents Chemother. 52: 2468-2472 [Abstract] [Full Text]  
  • Alastruey-Izquierdo, A., Cuenca-Estrella, M., Monzon, A., Mellado, E., Rodriguez-Tudela, J. L. (2008). Antifungal susceptibility profile of clinical Fusarium spp. isolates identified by molecular methods. J Antimicrob Chemother 61: 805-809 [Abstract] [Full Text]  
  • Alcazar-Fuoli, L., Mellado, E., Alastruey-Izquierdo, A., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2008). Aspergillus Section Fumigati: Antifungal Susceptibility Patterns and Sequence-Based Identification. Antimicrob. Agents Chemother. 52: 1244-1251 [Abstract] [Full Text]  
  • Garcia-Effron, G., Dilger, A., Alcazar-Fuoli, L., Park, S., Mellado, E., Perlin, D. S. (2008). Rapid Detection of Triazole Antifungal Resistance in Aspergillus fumigatus. J. Clin. Microbiol. 46: 1200-1206 [Abstract] [Full Text]  
  • Cuenca-Estrella, M., Alastruey-Izquierdo, A., Alcazar-Fuoli, L., Bernal-Martinez, L., Gomez-Lopez, A., Buitrago, M. J., Mellado, E., Rodriguez-Tudela, J. L. (2008). In Vitro Activities of 35 Double Combinations of Antifungal Agents against Scedosporium apiospermum and Scedosporium prolificans. Antimicrob. Agents Chemother. 52: 1136-1139 [Abstract] [Full Text]  
  • Antachopoulos, C., Meletiadis, J., Sein, T., Roilides, E., Walsh, T. J. (2008). Comparative In Vitro Pharmacodynamics of Caspofungin, Micafungin, and Anidulafungin against Germinated and Nongerminated Aspergillus Conidia. Antimicrob. Agents Chemother. 52: 321-328 [Abstract] [Full Text]  
  • Rodriguez-Tudela, J. L., Alcazar-Fuoli, L., Alastruey-Izquierdo, A., Monzon, A., Mellado, E., Cuenca-Estrella, M. (2007). Time of Incubation for Antifungal Susceptibility Testing of Aspergillus fumigatus: Can MIC Values Be Obtained at 24 Hours?. Antimicrob. Agents Chemother. 51: 4502-4504 [Abstract] [Full Text]  
  • Meletiadis, J., Antachopoulos, C., Stergiopoulou, T., Pournaras, S., Roilides, E., Walsh, T. J. (2007). Differential Fungicidal Activities of Amphotericin B and Voriconazole against Aspergillus Species Determined by Microbroth Methodology. Antimicrob. Agents Chemother. 51: 3329-3337 [Abstract] [Full Text]  
  • Mellado, E., Garcia-Effron, G., Alcazar-Fuoli, L., Melchers, W. J. G., Verweij, P. E., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2007). A New Aspergillus fumigatus Resistance Mechanism Conferring In Vitro Cross-Resistance to Azole Antifungals Involves a Combination of cyp51A Alterations. Antimicrob. Agents Chemother. 51: 1897-1904 [Abstract] [Full Text]  
  • Alastruey-Izquierdo, A., Cuenca-Estrella, M., Monzon, A., Rodriguez-Tudela, J. L. (2007). Prevalence and Susceptibility Testing of New Species of Pseudallescheria and Scedosporium in a Collection of Clinical Mold Isolates. Antimicrob. Agents Chemother. 51: 748-751 [Abstract] [Full Text]  
  • Cuenca-Estrella, M., Gomez-Lopez, A., Buitrago, M. J., Mellado, E., Garcia-Effron, G., Rodriguez-Tudela, J. L. (2006). In Vitro Activities of 10 Combinations of Antifungal Agents against the Multiresistant Pathogen Scopulariopsis brevicaulis.. Antimicrob. Agents Chemother. 50: 2248-2250 [Abstract] [Full Text]  
  • Cuenca-Estrella, M., Gomez-Lopez, A., Mellado, E., Buitrago, M. J., Monzon, A., Rodriguez-Tudela, J. L. (2006). Head-to-Head Comparison of the Activities of Currently Available Antifungal Agents against 3,378 Spanish Clinical Isolates of Yeasts and Filamentous Fungi. Antimicrob. Agents Chemother. 50: 917-921 [Abstract] [Full Text]  
  • Alcazar-Fuoli, L., Mellado, E., Garcia-Effron, G., Buitrago, M. J., Lopez, J. F., Grimalt, J. O., Cuenca-Estrella, J. M., Rodriguez-Tudela, J. L. (2006). Aspergillus fumigatus C-5 Sterol Desaturases Erg3A and Erg3B: Role in Sterol Biosynthesis and Antifungal Drug Susceptibility. Antimicrob. Agents Chemother. 50: 453-460 [Abstract] [Full Text]  
  • Cuenca-Estrella, M., Gomez-Lopez, A., Mellado, E., Garcia-Effron, G., Monzon, A., Rodriguez-Tudela, J. L. (2005). In Vitro Activity of Ravuconazole against 923 Clinical Isolates of Nondermatophyte Filamentous Fungi. Antimicrob. Agents Chemother. 49: 5136-5138 [Abstract] [Full Text]  
  • Mellado, E., Garcia-Effron, G., Buitrago, M. J., Alcazar-Fuoli, L., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2005). Targeted Gene Disruption of the 14-{alpha} Sterol Demethylase (cyp51A) in Aspergillus fumigatus and Its Role in Azole Drug Susceptibility. Antimicrob. Agents Chemother. 49: 2536-2538 [Abstract] [Full Text]  
  • Garcia-Effron, G., Mellado, E., Gomez-Lopez, A., Alcazar-Fuoli, L., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2005). Differences in Interactions between Azole Drugs Related to Modifications in the 14-{alpha} Sterol Demethylase Gene (cyp51A) of Aspergillus fumigatus. Antimicrob. Agents Chemother. 49: 2119-2121 [Abstract] [Full Text]  
  • Cuenca-Estrella, M., Gomez-Lopez, A., Garcia-Effron, G., Alcazar-Fuoli, L., Mellado, E., Buitrago, M. J., Rodriguez-Tudela, J. L. (2005). Combined Activity In Vitro of Caspofungin, Amphotericin B, and Azole Agents against Itraconazole-Resistant Clinical Isolates of Aspergillus fumigatus. Antimicrob. Agents Chemother. 49: 1232-1235 [Abstract] [Full Text]  
  • Gomez-Lopez, A., Aberkane, A., Petrikkou, E., Mellado, E., Rodriguez-Tudela, J. L., Cuenca-Estrella, M. (2005). Analysis of the Influence of Tween Concentration, Inoculum Size, Assay Medium, and Reading Time on Susceptibility Testing of Aspergillus spp.. J. Clin. Microbiol. 43: 1251-1255 [Abstract] [Full Text]  
  • Mellado, E., Garcia-Effron, G., Alcazar-Fuoli, L., Cuenca-Estrella, M., Rodriguez-Tudela, J. L. (2004). Substitutions at Methionine 220 in the 14{alpha}-Sterol Demethylase (Cyp51A) of Aspergillus fumigatus Are Responsible for Resistance In Vitro to Azole Antifungal Drugs. Antimicrob. Agents Chemother. 48: 2747-2750 [Abstract] [Full Text]  
  • Garcia-Effron, G., Gomez-Lopez, A., Mellado, E., Monzon, A., Rodriguez-Tudela, J. L., Cuenca-Estrella, M. (2004). In vitro activity of terbinafine against medically important non-dermatophyte species of filamentous fungi. J Antimicrob Chemother 53: 1086-1089 [Abstract] [Full Text]  


Copyright © 2009 by the American Society for Microbiology.   For an alternate route to Journals.ASM.org, visit: http://intl-journals.asm.org | More Info»