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Journal of Clinical Microbiology, November 2003, p. 5238-5239, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5238-5239.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Acridine Orange Staining as a Replacement of Subcultures for BacT/ALERT-Positive, Gram Stain-Negative Blood Cultures

Hildegard Adler,* Nathalie Baumlin, and Reno Frei

Bacteriology Laboratory, University of Basel Hospitals and Clinics, 4031 Basel, Switzerland

Received 13 June 2003/ Returned for modification 11 July 2003/ Accepted 20 August 2003

Among 18,424 blood culture sets processed during a study period of 18 months, 85 bottles that were positive by the BacT/ALERT system were Gram stain negative. Both acridine orange staining and subcultures detected microorganisms in a total of 12 bottles. Acridine orange staining can replace subcultures of false-positive blood cultures.

* Corresponding author. Mailing address: Bacteriology Laboratory, University of Basel Hospitals and Clinics, Petersgraben 4, 4031 Basel, Switzerland. Phone: 41-61-2654248. Fax: 41-61-2655355. E-mail: hadler{at}uhbs.ch.

Journal of Clinical Microbiology, November 2003, p. 5238-5239, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5238-5239.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.





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