This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Wadowsky, R. M. Articles by Rowe, D. Search for Related Content PubMed PubMed Citation Articles by Wadowsky, R. M. Articles by Rowe, D.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, November 2003, p. 5245-5249, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5245-5249.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Measurement of Epstein-Barr Virus DNA Loads in Whole Blood and Plasma by TaqMan PCR and in Peripheral Blood Lymphocytes by Competitive PCR

Departments of Pathology,¹ Pediatrics,⁴ Surgery, School of Medicine,⁵ Department of Infectious Diseases and Microbiology, Graduate School of Public Health, University of Pittsburgh,² Children's Hospital of Pittsburgh, Pittsburgh, Pennsylvania 15213³

Received 2 April 2003/ Returned for modification 19 July 2003/ Accepted 5 August 2003

Epstein-Barr virus (EBV) DNA load values were measured in samples of whole blood (n = 60) and plasma (n = 59) by TaqMan PCR and in samples of peripheral blood lymphocytes (PBLs) (n = 60) by competitive PCR (cPCR). The samples were obtained from 44 transplant recipients. The whole-blood and PBL loads correlated highly (r² > 0.900), whereas the plasma and PBL loads correlated poorly (r² = 0.512). Testing of whole blood by TaqMan PCR is an acceptable alternative to testing of PBLs by cPCR for quantifying EBV DNA load.

This article has been cited by other articles:

• Gulley, M. L., Tang, W. (2008). Laboratory Assays for Epstein-Barr Virus-Related Disease. J. Mol. Diagn. 10: 279-292 [Abstract] [Full Text]  
• Hayden, R. T., Hokanson, K. M., Pounds, S. B., Bankowski, M. J, Belzer, S. W., Carr, J., Diorio, D., Forman, M. S., Joshi, Y., Hillyard, D., Hodinka, R. L., Nikiforova, M. N., Romain, C. A., Stevenson, J., Valsamakis, A., Balfour, H. H. Jr., for the U.S. EBV Working Group, (2008). Multicenter Comparison of Different Real-Time PCR Assays for Quantitative Detection of Epstein-Barr Virus. J. Clin. Microbiol. 46: 157-163 [Abstract] [Full Text]  
• Hakim, H., Gibson, C., Pan, J., Srivastava, K., Gu, Z., Bankowski, M. J., Hayden, R. T. (2007). Comparison of Various Blood Compartments and Reporting Units for the Detection and Quantification of Epstein-Barr Virus in Peripheral Blood. J. Clin. Microbiol. 45: 2151-2155 [Abstract] [Full Text]  
• Wada, K., Kubota, N., Ito, Y., Yagasaki, H., Kato, K., Yoshikawa, T., Ono, Y., Ando, H., Fujimoto, Y., Kiuchi, T., Kojima, S., Nishiyama, Y., Kimura, H. (2007). Simultaneous Quantification of Epstein-Barr Virus, Cytomegalovirus, and Human Herpesvirus 6 DNA in Samples from Transplant Recipients by Multiplex Real-Time PCR Assay. J. Clin. Microbiol. 45: 1426-1432 [Abstract] [Full Text]  
• Espy, M. J., Uhl, J. R., Sloan, L. M., Buckwalter, S. P., Jones, M. F., Vetter, E. A., Yao, J. D. C., Wengenack, N. L., Rosenblatt, J. E., Cockerill, F. R. III, Smith, T. F. (2006). Real-Time PCR in Clinical Microbiology: Applications for Routine Laboratory Testing. Clin. Microbiol. Rev. 19: 165-256 [Abstract] [Full Text]  
• Stevens, S. J. C., Verkuijlen, S. A. W. M., Hariwiyanto, B., Harijadi, , Fachiroh, J., Paramita, D. K., Tan, I. B., Haryana, S. M., Middeldorp, J. M. (2005). Diagnostic Value of Measuring Epstein-Barr Virus (EBV) DNA Load and Carcinoma-Specific Viral mRNA in Relation to Anti-EBV Immunoglobulin A (IgA) and IgG Antibody Levels in Blood of Nasopharyngeal Carcinoma Patients from Indonesia. J. Clin. Microbiol. 43: 3066-3073 [Abstract] [Full Text]