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Journal of Clinical Microbiology, November 2003, p. 5254-5257, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5254-5257.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Surveillance of Cryptosporidium spp. in Raw Wastewater in Milwaukee: Implications for Understanding Outbreak Occurrence and Transmission Dynamics

Ling Zhou,1 Ajaib Singh,2 Jianlin Jiang,1 and Lihua Xiao1*

Division of Parasitic Diseases, Centers for Disease Control and Prevention, Atlanta, Georgia 30341,1 City of Milwaukee Public Health Laboratories, Milwaukee, Wisconsin 532022

Received 5 May 2003/ Returned for modification 16 July 2003/ Accepted 20 August 2003

Six Cryptosporidium spp. were found in 50 of 179 Milwaukee wastewater samples collected weekly over a year. Of the eight subtypes of Cryptosporidium hominis and Cryptosporidium parvum present, allele Ib was found in 14 of 16 samples, and its sequence was identical to that of the subtype in human samples from the 1993 Milwaukee outbreak of cryptosporidiosis.

* Corresponding author. Mailing address: Division of Parasitic Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Building 22, Mail Stop F-12, 4770 Buford Hwy., Atlanta, GA 30341-3717. Phone: (770) 488-4840. Fax: (770) 488-4454. E-mail: lxiao{at}cdc.gov.

Journal of Clinical Microbiology, November 2003, p. 5254-5257, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5254-5257.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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