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Journal of Clinical Microbiology, November 2003, p. 5270-5272, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5270-5272.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of Etest with Modified Broth Microdilution Method for Testing Susceptibility of Aspergillus spp. to Voriconazole

M. C. Serrano,1 D. Morilla,1 A. Valverde,1 M. Chávez,1 A. Espinel-Ingroff,2 R. Claro,1 M. Ramírez,1 and E. Martín Mazuelos1*

Servicio de Microbiología Clínica, Hospital Universitario de Valme, Seville 41014, Spain,1 Division of Infectious Diseases, Medical College of Virginia, Virginia Commonwealth University, Richmond, Virginia 239802

Received 22 January 2003/ Returned for modification 30 March 2003/ Accepted 21 July 2003

We compared the Etest with a broth microdilution method, performed according to a modified National Committee for Clinical Laboratory Standards guideline (M38-A), for determining the in vitro susceptibility of 77 isolates of Aspergillus spp. (26 A. fumigatus, 21 A. flavus, 10 A. terreus, 9 A. niger, 5 A. nidulellus, 4 A. glaucus, and 2 A. flavipes isolates). Overall, there was 92.2% agreement between both methods when Etest MICs were read at 24 h and 83.1% agreement when both methods were read at 48 h. When Etest MICs were read at 24 h, the agreement was >90% for all species tested except for A. fumigatus (84.6%). When Etest MICs were read at 48 h, the agreement ranged from 50 to 100%. The poorest agreement was seen with A. glaucus (50%) and A. fumigatus (65%). Where a discrepancy was observed between Etest and the reference method, the Etest MIC was generally higher. The Etest appears to be a suitable alternative procedure for testing the susceptibility of Aspergillus spp. to voriconazole.

* Corresponding author. Mailing address: H.U. Valme, Ctra. Cádiz s/n, Seville 41014, Spain. Phone: 034-955015480. Fax: 034-955015481. E-mail: estrella.martin.sspa{at}juntadeandalucia.es.

Journal of Clinical Microbiology, November 2003, p. 5270-5272, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5270-5272.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Castro, C., Serrano, M. C., Flores, B., Espinel-Ingroff, A., Martin-Mazuelos, E. (2004). Comparison of the Sensititre YeastOne Colorimetric Antifungal Panel with a Modified NCCLS M38-A Method To Determine the Activity of Voriconazole against Clinical Isolates of Aspergillus spp.. J. Clin. Microbiol. 42: 4358-4360 [Abstract] [Full Text]  


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