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Journal of Clinical Microbiology, November 2003, p. 5273-5276, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5273-5276.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Efficient Extraction of Viral DNA and Viral RNA by the Chemagic Viral DNA/RNA Kit Allows Sensitive Detection of Cytomegalovirus, Hepatitis B Virus, and Hepatitis G Virus by PCR

Michael Kleines, Kirsten Schellenberg, and Klaus Ritter*

Division of Virology, Department of Medical Microbiology, University Hospital RWTH Aachen, Aachen, Germany

Received 27 May 2003/ Returned for modification 14 July 2003/ Accepted 17 August 2003

The Chemagic Viral DNA/RNA kit was evaluated for extraction of cytomegalovirus (CMV), hepatitis B virus (HBV), and hepatitis G virus (HGV) by using the QIAamp DNA Blood Mini kit and the QIAamp Viral RNA Mini kit as reference protocols. The extraction efficiencies of the different kits for CMV DNA and HBV DNA were not distinguishable, but the extraction efficiency for HGV RNA was better with the Chemagen protocol. All clinical specimens tested HBV DNA- or HGV RNA-positive after QIAGEN protocols for extraction were confirmed by using the Chemagen protocol. The Chemagen kit failed to confirm one of 75 CMV DNA-positive specimens. Thus, a new competitive extraction method using a technology with a high potential for automation is available.

* Corresponding author. Mailing address: Division of Virology, University Hospital Aachen, Pauwelsstrasse 30, D-52074 Aachen, Germany. Phone: 49 241 8088460. Fax: 49 241 8082483. E-mail: kritter{at}ukaachen.de.

Journal of Clinical Microbiology, November 2003, p. 5273-5276, Vol. 41, No. 11
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.11.5273-5276.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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