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Journal of Clinical Microbiology, December 2003, p. 5537-5540, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5537-5540.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Listeria monocytogenes Serotype Identification by PCR

Monica K. Borucki1,2* and Douglas R. Call2

Animal Disease Research Unit, Agricultural Research Service, U.S. Department of Agriculture, Pullman, Washington 99164-6630,1 Department of Veterinary Microbiology and Pathology, College of Veterinary Medicine, Washington State University, Pullman, Washington 99164-70402

Received 11 June 2003/ Returned for modification 8 August 2003/ Accepted 28 August 2003

Serotyping is a universally accepted subtyping method for Listeria monocytogenes. Identification of the strain serotype permits differentiation between important food-borne strains (1/2a, 1/2b, and 4b) and provides a "gold standard" for comparing isolates analyzed in different labs and with different techniques. Although an efficient enzyme-linked immunosorbent assay serotyping protocol was described recently, identification of PCR serotyping primers would further increase the ease and accessibility of this classification system. Serotyping PCR primers were designed from variable regions of the L. monocytogenes genome. Three primer sets were used in conjunction with a previously described Division III primer set in order to classify 122 L. monocytogenes strains into five serotype groups [1/2a(3a), 1/2b, 1/2c(3c), 4b(d,e), and 4a/c]. Results of the PCR method agreed with those of the conventional slide agglutination method for 97, 100, 94, and 91% of strains belonging to serotypes 1/2a, 1/2b, 1/2c, and 4b, respectively.

* Corresponding author. Mailing address: USDA Animal Research Service, 3003 ADBF, Washington State University, Pullman, WA 99164-6630. Phone: (509) 335-7407. Fax: (509) 335-8328. E-mail: mborucki{at}vetmed.wsu.edu.

Journal of Clinical Microbiology, December 2003, p. 5537-5540, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5537-5540.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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