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Journal of Clinical Microbiology, December 2003, p. 5726-5728, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5726-5728.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

New Reverse Transcription-PCR Assay for Rapid and Sensitive Detection of Enterovirus Genomes in Cerebrospinal Fluid Specimens of Patients with Aseptic Meningitis

Jérôme Jacques,1 Jocelyne Carquin,1 Véronique Brodard,1 Hélène Moret,1 Dorine Lebrun,1 Maude Bouscambert,1 Jacques Motte,2 Gérard Rémy,3 and Laurent Andréoletti1*

Laboratoire de Virologie, Centre Hospitalier Universitaire, IFR 53/EA-3309 Faculté de Médecine de Reims,1 Service des Urgences Médico-Chirurgicales Pédiatriques et Faculté de Médecine de Reims,2 Service de Médecine Interne et des Maladies Infectieuses, Hôpital Robert Debré CHU de Reims, Reims, France3

Received 5 June 2003/ Returned for modification 14 July 2003/ Accepted 31 August 2003

Enterovirus (EV) detection by a new commercially available reverse transcription (RT)-PCR assay (Penter RT-PCR test) was compared with EV isolation from cell cultures and with EV detection by an in-house RT-PCR assay. Of the 54 cerebrospinal fluid specimens collected during a summer outbreak of aseptic meningitis, 52% were positive by cell culture versus 76% by in-house RT-PCR assay and 80% by the new RT-PCR test (52 versus 76 versus 80%; P = 0.003). This new reliable EV RNA detection test is suitable for clinical diagnosis of EV-related meningitis and may improve the management of EV-related neurological syndromes.

* Corresponding author. Mailing address: Laboratoire de Virologie, Service de Microbiologie, CHU de Reims, Avenue du Général Koenig, 51092 Reims Cedex, France. Phone: (33) 3 26 78 39 93. Fax: (33) 3 26 78 41 34. E-mail: landreoletti{at}chu-reims.fr.

Journal of Clinical Microbiology, December 2003, p. 5726-5728, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5726-5728.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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