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Journal of Clinical Microbiology, December 2003, p. 5735-5737, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5735-5737.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Comparison of the Sequences of the Internal Transcribed Spacer Regions and PbGP43 Genes of Paracoccidioides brasiliensis from Patients and Armadillos (Dasypus novemcinctus)

Flavia Hebeler-Barbosa,1 Flavia V. Morais,2 Mario R. Montenegro,3 Eiko E. Kuramae,4 Beatriz Montes,5 Juan G. McEwen,5 Eduardo Bagagli,1 and Rosana Puccia2*

Departamento de Microbiologia e Imunologia, Instituto de Biociências,1 Departamento de Patologias, Universidade Estadual Paulista, Botucatu,3 Departamento de Microbiologia, Imunologia e Parasitologia, Universidade Federal de São Paulo, São Paulo, Brazil,2 Fungal Biodiversity Center, CBS, Utrecht, The Netherlands,4 Corporacion para Investigaciones Biologicas and Facultad de Medicina, Universidad de Antioquia, Medellin, Colombia5

Received 17 June 2003/ Returned for modification 7 August 2003/ Accepted 13 September 2003

Paracoccidioides brasiliensis isolates from 10 nine-banded armadillos (Dasypus novemcinctus) were comparable with 19 clinical isolates by sequence analysis of the PbGP43 gene and ribosomal internal transcribed spacer 1 (ITS1) and ITS2 and by random amplified polymorphic DNA. In this original ITS study, eight isolates differed by one or three sites among five total substitution sites.


* Corresponding author. Mailing address: Disciplina de Biologia Celular, UNIFESP, Rua Botucatu, 862, oitavo andar, São Paulo, SP, 04023-062 Brasil. Phone: 55-11-5084-2991. Fax: 55-11-5571-5877. E-mail: rosana{at}ecb.epm.br.


Journal of Clinical Microbiology, December 2003, p. 5735-5737, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5735-5737.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.




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