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Journal of Clinical Microbiology, December 2003, p. 5742-5746, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5742-5746.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Enterococcal Surface Protein Gene (esp) and Amplified Fragment Length Polymorphism Typing of Glycopeptide-Resistant Enterococcus faecium during Its Emergence in a Greek Intensive Care Unit

C. Routsi,1* E. Platsouka,2 R. J. L. Willems,3 M. J. M. Bonten,4 O. Paniara,2 G. Saroglou,1 and C. Roussos1

Department of Critical Care, Medical School of Athens University,1 Department of Clinical Microbiology, Evangelismos Hospital, Athens, Greece,2 Diagnostic Laboratory for Infectious Diseases and Perinatal Screening, National Institute of Public Health and the Environment, Bilthoven,3 Department of Internal Medicine, University Medical Center, Utrecht, The Netherlands4

Received 8 May 2003/ Returned for modification 23 June 2003/ Accepted 5 September 2003

The emergence of glycopeptide-resistant Enterococcus faecium (GREF) in a Greek intensive care unit was studied by amplified fragment length polymorphism analysis and esp gene detection. Three GREF clones harboring the esp gene were recovered from 17 out of 21 patients, indicating the dissemination of genetically homogenous and virulent strains of GREF.

* Corresponding author. Mailing address: Evangelismos Hospital, Critical Care Department, 45-47 Ipsilantou Str., Athens 106 75, Greece. Phone: 210 7243320. Fax: 210 7244941. E-mail: croutsi{at}hotmail.com.

Journal of Clinical Microbiology, December 2003, p. 5742-5746, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5742-5746.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Top, J., Schouls, L. M., Bonten, M. J. M., Willems, R. J. L. (2004). Multiple-Locus Variable-Number Tandem Repeat Analysis, a Novel Typing Scheme To Study the Genetic Relatedness and Epidemiology of Enterococcus faecium Isolates. J. Clin. Microbiol. 42: 4503-4511 [Abstract] [Full Text]  


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