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Journal of Clinical Microbiology, December 2003, p. 5750-5754, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5750-5754.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Comparison of Isolates of Burkholderia multivorans from Patients with Cystic Fibrosis in the United Kingdom

Health Protection Agency, Laboratory of HealthCare Associated Infection, London NW9 5HT, United Kingdom

Received 2 June 2003/ Returned for modification 22 July 2003/ Accepted 22 August 2003

Burkholderia multivorans strains from 47 cystic fibrosis (CF) patients in 28 hospitals were compared by pulsed-field gel electrophoresis (PFGE) and flagellin (fliC) PCR-restriction fragment length polymorphism (PCR-RFLP) analysis. A considerable degree of genetic variation was evident, with each patient harboring a strain with a unique PFGE profile. Four sizes of fliC amplicons were produced, and these amplicons gave 13 RFLP types with restriction enzyme MspI. B. multivorans did not appear to spread between patients, suggesting that most CF patients acquire the organism from the natural environment.

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