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Journal of Clinical Microbiology, December 2003, p. 5760-5763, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5760-5763.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of Enzyme-Linked Immunosorbent Assays and a PCR Test for Detection of Shiga Toxins for Shiga Toxin-Producing Escherichia coli in Cattle Herds

Monserrat Segura-Alvarez,1 Heinz Richter,2 Franz J. Conraths,1 and Lutz Geue1*

Federal Research Centre for Virus Diseases of Animals, Institute of Epidemiology, D-16868 Wusterhausen,1 Bundesinstitut für Risikobewertung, D-06846 Dessau, Germany2

Received 4 April 2003/ Returned for modification 26 May 2003/ Accepted 28 August 2003

Antigen capture enzyme-linked immunosorbent assays (ELISAs) for the detection of Stx1 and/or Stx2 in cattle feces were validated in comparison to the Vero cell cytotoxicity neutralization test (as a "gold standard") applied in the course of a monitoring program for Shiga toxin-producing Escherichia coli in German cattle herds as a prescreening test and compared to MK1/MK2 PCR as an alternative prescreening test.

* Corresponding author. Mailing address: Federal Research Centre for Virus Diseases of Animals, Institute of Epidemiology, Seestrasse 55, D-16868 Wusterhausen, Germany. Phone: 49 33979 80189. Fax: 49 33979 80222. E-mail: lutz.geue{at}wus.bfav.de.

Journal of Clinical Microbiology, December 2003, p. 5760-5763, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5760-5763.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Copyright © 2003 by the American Society for Microbiology. All rights reserved.