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Journal of Clinical Microbiology, December 2003, p. 5764-5769, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5764-5769.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Serologic and Genomic Characterization of a G12 Human Rotavirus in Thailand

Department of Virology and Parasitology, Fujita Health University School of Medicine, Toyoake, Aichi 470-1192,¹ Department of Hygiene, Sapporo Medical University School of Medicine, Sapporo 060-8556, Japan,² Enteric and Respiratory Viruses Laboratory, National Institute of Health, Department of Medical Sciences, Nonthaburi 11000, Thailand³

Received 9 December 2002/ Returned for modification 5 May 2003/ Accepted 14 September 2003

The G and P type specificity of the human rotavirus strain T-152 (G12P[9]) isolated in Thailand was serologically confirmed with G12-specific monoclonal antibodies prepared in this study by using a reference G12 strain, L26, as an immunizing antigen and a P[9]-specific monoclonal antibody, respectively. The genomic relationship of strain T-152 with representative human rotavirus strains was examined by means of Northern blot analysis. The results showed that T152 is closely related to strain AU-1 (G3P[9]). Gene 5 (NSP1 gene) of T152, which did not hybridize with those of any other strains examined, was characterized by sequence determination. The T152 NSP1 gene is 1,652 nucleotides in length, encodes 493 amino acids, and exhibits low identity to those of representative human and animal rotaviruses.

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