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Journal of Clinical Microbiology, December 2003, p. 5774-5777, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5774-5777.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Evaluation of a PCR-Based Universal Heteroduplex Generator Assay as a Tool for Rapid Detection of Multidrug-Resistant Mycobacterium tuberculosis in Peru

Holger Mayta,1,2 Robert H. Gilman,1,2,3* Fanny Arenas,2 Teresa Valencia,2 Luz Caviedes,2 Sonia H. Montenegro,4 Eduardo Ticona,5 Jaime Ortiz,6 Rosa Chumpitaz,2 Carlton A. Evans,7 Diana L. Williams,8 and The Tuberculosis Working Group in Perú{dagger}

Department of International Health, Johns Hopkins Bloomberg School of Public Health, Baltimore, Maryland,1 Department of Microbiology, Universidad Peruana Cayetano Heredia,2 Asociación Benefica Proyectos en Informatica, Salud, Medicina y Agricultura,3 Dos de Mayo Hospital,5 Maria Auxiliadora Hospital, Lima, Perú,6 Department of Medicine, Imperial College, London, United Kingdom,7 Ochsner Medical Foundation, New Orleans,4 Laboratory Research Branch, Molecular Biology Research, National Hansen's Disease Program LSU-SVM, Baton Rouge, Louisiana8

Received 14 May 2003/ Returned for modification 11 July 2003/ Accepted 31 August 2003

Multidrug-resistant tuberculosis is an increasing health problem worldwide, especially in developing countries. The PCR-UHG-Rif assay, which detects mutations within the rpoB gene associated with rifampin resistance, was evaluated for its ability and reliability to detect and identify drug-resistant Mycobacterium tuberculosis in a developing country where tuberculosis is highly endemic.

* Corresponding author. Mailing address: Department of International Health, Johns Hopkins University, School of Public Health, 615 N. Wolfe St. Rm. W3501, Baltimore, MD 21205. Phone: (410) 614-3959. Fax: (410) 614-6060. E-mail: Rgilman{at}jhsph.edu.

{dagger} Members of The Tuberculosis Working Group in Peru are listed in Acknowledgments.

Journal of Clinical Microbiology, December 2003, p. 5774-5777, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5774-5777.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

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