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Journal of Clinical Microbiology, December 2003, p. 5778-5780, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5778-5780.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

False Identification of Coccidioides immitis: Do Molecular Methods Always Get It Right?

B. Cherie Millar,1 Xu Jiru,1 Michael J. Walker,2 James P. Evans,2 and John E. Moore1*

Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD,1 Regional Mycology Reference Laboratory, The Royal Group of Hospitals, Belfast BT12 6BA, Northern Ireland, United Kingdom2

Received 17 February 2003/ Returned for modification 17 April 2003/ Accepted 13 May 2003

rRNA sequence analysis of a partial region of the 18S and 5.8S-internal transcribed spacer 2 (ITS2) region of Chrysosporium keratinophilum highlights its potential molecular misidentification as Coccidioides immitis. Molecular identification of medically important fungi should not be based solely on sequence analysis of the 18S rRNA gene but should be confirmed by sequence analysis of an additional rRNA gene locus, such as the ITS region(s).

* Corresponding author. Mailing address: Northern Ireland Public Health Laboratory, Department of Bacteriology, Belfast City Hospital, Belfast BT9 7AD, Northern Ireland, United Kingdom. Phone: 44 28 9026 3554. Fax: 44 28 2589 2887. E-mail: jemoore{at}niphl.dnet.co.uk.

Journal of Clinical Microbiology, December 2003, p. 5778-5780, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5778-5780.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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