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Journal of Clinical Microbiology, December 2003, p. 5813-5816, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5813-5816.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Detection and Identification of Agents of Eumycetoma: Detailed Report of Two Cases

Abdalla O. A. Ahmed,1,2 Nicole Desplaces,3 Philippe Leonard,4 Fred Goldstein,5 Sybren De Hoog,6 Henri Verbrugh,2 and Alex van Belkum2*

Mycetoma Research Group, Institute of Endemic Diseases, University of Khartoum, Khartoum, Sudan,1 Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Rotterdam,2 Centraalbureau voor Schimmelcultures (CBS), Utrecht, The Netherlands,6 Department of Microbiology,3 Department of Orthopedic Surgery, Croix Saint Simon Hospital,4 Department of Microbiology, Saint Joseph Hospital, Paris, France5

Received 17 June 2003/ Returned for modification 7 August 2003/ Accepted 22 August 2003

We describe two cases of eumycetoma in the legs. The infections could not be adequately diagnosed by classical mycology, but the causative agents were successfully identified as Madurella mycetomatis by species-specific PCR and DNA sequencing.

* Corresponding author. Mailing address: Department of Medical Microbiology and Infectious Diseases, Erasmus MC, Dr. Molewaterplein 40, 3015 GD Rotterdam, The Netherlands. Phone: 31 10 4635813. Fax: 31 10 4633875. E-mail: a.vanbelkum{at}erasmusmc.nl.

Journal of Clinical Microbiology, December 2003, p. 5813-5816, Vol. 41, No. 12
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.12.5813-5816.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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  • Ahmed, A. O. A., van de Sande, W. W. J., van Vianen, W., van Belkum, A., Fahal, A. H., Verbrugh, H. A., Bakker-Woudenberg, I. A. J. M. (2004). In Vitro Susceptibilities of Madurella mycetomatis to Itraconazole and Amphotericin B Assessed by a Modified NCCLS Method and a Viability-Based 2,3-Bis(2-Methoxy-4-Nitro-5- Sulfophenyl)-5-[(Phenylamino)Carbonyl]-2H- Tetrazolium Hydroxide (XTT) Assay. Antimicrob. Agents Chemother. 48: 2742-2746 [Abstract] [Full Text]  


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