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Journal of Clinical Microbiology, February 2003, p. 547-551, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.547-551.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification of a Specific Antigenic Region of the P82 Protein of Babesia equi and Its Potential Use in Serodiagnosis

Haruyuki Hirata, Xuenan Xuan, Naoaki Yokoyama, Yoshifumi Nishikawa, Kozo Fujisaki, Naoyoshi Suzuki, and Ikuo Igarashi*

National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada-cho, Obihiro, Hokkaido 080-8555, Japan

Received 11 July 2002/ Returned for modification 21 August 2002/ Accepted 31 October 2002

The efficacy of the Be82 gene product fused with glutathione S-transferase (GST/Be82) in an enzyme-linked immunosorbent assay (ELISA) for the diagnosis of Babesia equi infection was reported previously (H. Hirata et al., J. Clin. Microbiol. 40:1470-1474, 2002). However, the ELISA with the GST/Be82 antigen cross-reacted with Babesia caballi-infected horse sera, despite the high rate of detection of B. equi. These results suggested that GST/Be82 has an antigen in common with B. caballi or antigenicity similar to that of B. caballi. In the present study, we constructed a series of five clones with deletions in the Be82 gene product, each of which was fused with GST, and used them in ELISAs in order to overcome the cross-reactivity seen with B. caballi. One of the deletion clones, a clone with a deletion of the Be82 gene from positions 236 to 381 (Be82/236-381), specifically and sensitively detected B. equi-infected horse sera without cross-reactivity with B. caballi-infected horse sera. Assays with clones from which other gene products were deleted showed decreased sensitivities or remained nonspecific for the detection of B. equi-infected horse sera. These results suggest that the Be82/236-381 gene product is a novel antigen for the diagnosis of B. equi infection in horses.

* Corresponding author. Mailing address: National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2-13, Inada-cho, Obihiro, Hokkaido 080-8555, Japan. Phone: 81-155-49-5641 Fax: 81-155-49-5643. E-mail: igarcpmi{at}obihiro.ac.jp.

Journal of Clinical Microbiology, February 2003, p. 547-551, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.547-551.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



This article has been cited by other articles:

  • Tsuji, N., Battsetseg, B., Boldbaatar, D., Miyoshi, T., Xuan, X., Oliver, J. H. Jr., Fujisaki, K. (2007). Babesial Vector Tick Defensin against Babesia sp. Parasites. Infect. Immun. 75: 3633-3640 [Abstract] [Full Text]  
  • Hirata, H., Yokoyama, N., Xuan, X., Fujisaki, K., Suzuki, N., Igarashi, I. (2005). Cloning of a Novel Babesia equi Gene Encoding a 158-Kilodalton Protein Useful for Serological Diagnosis. CVI 12: 334-338 [Abstract] [Full Text]  
  • Tamaki, Y., Hirata, H., Takabatake, N., Bork, S., Yokoyama, N., Xuan, X., Fujisaki, K., Igarashi, I. (2004). Molecular Cloning of a Babesia caballi Gene Encoding the 134-Kilodalton Protein and Evaluation of Its Diagnostic Potential in an Enzyme-Linked Immunosorbent Assay. CVI 11: 211-215 [Abstract] [Full Text]  


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