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Journal of Clinical Microbiology, February 2003, p. 564-571, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.564-571.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Serotyping of Listeria monocytogenes by Enzyme-Linked Immunosorbent Assay and Identification of Mixed-Serotype Cultures by Colony Immunoblotting

Jeffrey D. Palumbo,1 Monica K. Borucki,2 Robert E. Mandrell,1 and Lisa Gorski1*

Produce Safety and Microbiology Research Unit, Agricultural Research Service, United States Department of Agriculture, Albany, California 94710-1105,1 Animal Disease Research Unit, Agricultural Research Service, United States Department of Agriculture, Pullman, Washington 99164-66302

Received 28 August 2002/ Returned for modification 17 October 2002/ Accepted 12 November 2002

Routine analysis of Listeria monocytogenes by serotyping using traditional agglutination methods is limited in use because of the expense and limited availability of commercially prepared antisera and intra- and interlaboratory discrepancies arising from differences in antiserum preparation and visual determination of agglutination. We have adapted a commercially available set of L. monocytogenes antisera to an enzyme-linked immunosorbent assay (ELISA) format for high-throughput, low-cost serotype determination. Rather than subjective visualization of agglutination, positive antigen and antiserum reactions were scored by a quantitative, colorimetric reaction. ELISA serotyping of 89 of 101 L. monocytogenes isolates agreed with slide agglutination serotyping data, and 100 previously uncharacterized isolates were serotyped unambiguously by the ELISA method. In addition, mixed-serotype cultures of L. monocytogenes were identified by a colony immunoblot procedure, in which serogroup 1/2 and serogroup 4 colonies were discriminated by differential staining.

* Corresponding author. Mailing address: USDA/ARS, WRRC, Produce Safety and Microbiology Research Unit, 800 Buchanan St., Albany, CA 94710. Phone: (510) 559-6046. Fax: (510) 559-6162. E-mail: lgorski{at}pw.usda.gov.

Journal of Clinical Microbiology, February 2003, p. 564-571, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.564-571.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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