This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Ravelo, C. Articles by Romalde, J. L. Search for Related Content PubMed PubMed Citation Articles by Ravelo, C. Articles by Romalde, J. L.

 Previous Article  |  Next Article 

Journal of Clinical Microbiology, February 2003, p. 751-756, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.751-756.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Molecular Fingerprinting of Fish-Pathogenic Lactococcus garvieae Strains by Random Amplified Polymorphic DNA Analysis

Departamento de Microbiología y Parasitología, Facultad de Biología, Universidad de Santiago de Compostela, 15782 Santiago de Compostela, Spain

Received 1 August 2002/ Returned for modification 22 October 2002/ Accepted 22 November 2002

In this work, we used the random amplified polymorphic DNA (RAPD) technique to evaluate the genetic diversity in Lactococcus garvieae, an important pathogen for fish. Fifty-seven strains with different hosts and geographical origins, including Japan and several countries of the Mediterranean area such as Spain, Portugal, France, Italy, England, and Turkey, were analyzed. Two primers, oligonucleotides 5 and 6 (Pharmacia Biotech) were utilized; primer 5 was the most discriminative, since allowed us to differentiate 10 RAPD -types related to the origin of the strains. Regardless of the oligonucleotide primer employed, the 57 isolates of L. garvieae studied were separated into three genetic groups, composed of the Spanish, Portuguese, English, and Turkish strains (group A), the Italian and French strains (group B), and the Japanese strains (group C). The similarity of isolates within each group, estimated on the basis of the Dice coefficient, ranged from 75 to 100%. Our findings also indicate that RAPD profiling constitutes a useful tool for epidemiological studies of this fish pathogen.

This article has been cited by other articles:

• Menendez, A., Fernandez, L., Reimundo, P., Guijarro, J. A. (2007). Genes required for Lactococcus garvieae survival in a fish host. Microbiology 153: 3286-3294 [Abstract] [Full Text]  
• El-Baradei, G., Delacroix-Buchet, A., Ogier, J.-C. (2007). Biodiversity of Bacterial Ecosystems in Traditional Egyptian Domiati Cheese. Appl. Environ. Microbiol. 73: 1248-1255 [Abstract] [Full Text]  
• Rantsiou, K., Urso, R., Iacumin, L., Cantoni, C., Cattaneo, P., Comi, G., Cocolin, L. (2005). Culture-Dependent and -Independent Methods To Investigate the Microbial Ecology of Italian Fermented Sausages. Appl. Environ. Microbiol. 71: 1977-1986 [Abstract] [Full Text]  
• Eyngor, M., Zlotkin, A., Ghittino, C., Prearo, M., Douet, D.-G., Chilmonczyk, S., Eldar, A. (2004). Clonality and Diversity of the Fish Pathogen Lactococcus garvieae in Mediterranean Countries. Appl. Environ. Microbiol. 70: 5132-5137 [Abstract] [Full Text]