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Journal of Clinical Microbiology, February 2003, p. 822-825, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.822-825.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Spread of Integron-Associated VIM-Type Metallo-ß-Lactamase Genes among Imipenem-Nonsusceptible Pseudomonas aeruginosa Strains in Greek Hospitals

P. Giakkoupi,1,2 G. Petrikkos,2 L. S. Tzouvelekis,3 S. Tsonas,1 The WHONET GREECE Study Group,,3,{dagger} N. J. Legakis,3 and A. C. Vatopoulos1*

Department of Hygiene & Epidemiology,1 Infectious Diseases and Antimicrobial Chemotherapy Research Laboratory "G.K. Daikos,",2 Department of Microbiology, Medical School, Athens University, Greece3

Received 6 June 2002/ Returned for modification 12 August 2002/ Accepted 12 November 2002

Fifty-eight imipenem-nonsusceptible (MIC >= 8 µg/ml) Pseudomonas aeruginosa strains isolated during May 2001 in 15 Greek hospitals were studied. Thirty-six isolates derived from nine hospitals carried VIM-type metallo-ß-lactamase genes, as found by PCR. In 34 isolates, blaVIM was associated with class 1 integrons of various sizes. DNA sequencing indicated the presence of blaVIM-2 gene cassettes in a variety of integron structures. Random amplified polymorphic DNA typing suggested diversity of the blaVIM-positive strains. Synergy between 2-mercaptoacetic acid and imipenem indicated carbapenemase activity in 26 blaVIM-positive strains.

* Corresponding author. Mailing address: Department of Hygiene & Epidemiology, Medical School, Athens University, 115 27 Athens (Goudi), Greece. Phone: 302107462071. Fax: 302107462080. E-mail: avatopou{at}med.uoa.gr.

{dagger} A list of participants in the WHONET-GREECE Study Group can be found at www.mednet.gr/whonet.

Journal of Clinical Microbiology, February 2003, p. 822-825, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.822-825.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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