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Journal of Clinical Microbiology, February 2003, p. 831-834, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.831-834.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Detection of Candida albicans mRNA from Formalin-Fixed, Paraffin-Embedded Mouse Tissues by Nested Reverse Transcription-PCR

David A. Schofield,* Caroline Westwater, Emily E. Paulling, Peter J. Nicholas, and Edward Balish

Division of Mycology, Department of Microbiology and Immunology, Medical University of South Carolina, Charleston, South Carolina 29403

Received 8 August 2002/ Returned for modification 31 October 2002/ Accepted 21 November 2002

Histopathology archives represent a vast source of infectious disease specimens that can be used to elucidate important disease processes. In this report, we describe a method to detect Candida albicans gene expression from infected, formalin-fixed, paraffin-embedded mouse tissue. By use of glass beads to break open the fungal cells and proteinase K treatment, RNA was extracted routinely from tissue sections that had been fixed for up to 72 h. Upon reverse transcription of the RNA and nested PCR, the procedure detected C. albicans "housekeeping" and putative virulence genes.

* Corresponding author. Mailing address: Department of Microbiology and Immunology, BSB 201, Medical University of South Carolina, 173 Ashley Ave., Charleston, SC 29403. Phone: (843) 792-7703. Fax: (843) 792-2464. E-mail: schofida{at}musc.edu.

Journal of Clinical Microbiology, February 2003, p. 831-834, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.831-834.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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