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Evaluation of the Specificities of Five DNA Amplification Methods for the Detection of Neisseria gonorrhoeae

Genitourinary Infections Reference Laboratory, Public Health Laboratory, Bristol Royal Infirmary, Bristol, BS2 8HW,¹ Liverpool Public Health Laboratory, University Hospital at Aintree, Liverpool, L9 7AL, United Kingdom²

Received 19 June 2002/ Returned for modification 14 October 2002/ Accepted 19 November 2002

The intragenus specificities of five molecular diagnostic methods for Neisseria gonorrhoeae were determined. Three assays were considered suboptimal. Molecular detection of N. gonorrhoeae from sites where other Neisseria spp. commonly occur or from any site in low-prevalence settings should be confirmed by a test targeting a different genetic locus.

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