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Journal of Clinical Microbiology, February 2003, p. 873-876, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.873-876.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Rapid Identification of the Genus Fonsecaea by PCR with Specific Oligonucleotide Primers

Paride Abliz, Kazutaka Fukushima,* Kayoko Takizawa, Norikazu Nieda, Makoto Miyaji, and Kazuko Nishimura

Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan

Received 26 February 2002/ Returned for modification 8 August 2002/ Accepted 15 November 2002

An oligonucleotide primer set based on internal transcribed spacer regions of ribosomal DNA for PCR which gives the amplicon for only the DNA from Fonsecaea species was designed. This set yielded an amplicon with 333 bp for all strains of Fonsecaea pedrosoi and Fonsecaea compacta examined but no amplicons for related dematiaceous fungi and pathogenic yeasts. PCR using this primer set was considered to be a useful method for the rapid identification of the genus Fonsecaea.

* Corresponding author. Mailing address: Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, 260-8673 Chiba, Japan. Phone: 81-43-226-2797. Fax: 81-43-226-2797 or 2486. E-mail: kfuky{at}myco.pf.chiba-u.ac.jp.

Journal of Clinical Microbiology, February 2003, p. 873-876, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.873-876.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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