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Journal of Clinical Microbiology, February 2003, p. 880-882, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.880-882.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Value of Indirect Hemagglutination and Coagglutination Tests for Serotyping Haemophilus parasuis

M. L. Del Río, C.B. Gutiérrez, and E. F. Rodríguez Ferri*

Section of Microbiology and Immunology, Department of Animal Health, Faculty of Veterinary Medicine, León, Spain

Received 5 August 2002/ Returned for modification 23 October 2002/ Accepted 22 November 2002

An indirect hemagglutination test (IHA) and a coagglutination test (CA) were evaluated using saline, boiled, and autoclaved extracts for serotyping Haemophilus parasuis. CA showed several cross-reactions, whereas IHA gave rise to specific reactions, with minor exceptions. IHA was further compared with the immunodiffusion test (the "gold standard") for the serotyping of 67 field isolates. As a conclusion, IHA is recommended as a useful method for sensitive and specific serotyping of H. parasuis.

* Corresponding author. Mailing address: Facultad de Veterinaria, Departamento de Sanidad Animal, Universidad de León, 24007-León, Spain. Phone: 34 987 291 297. Fax: 34 987 291 304. E-mail: dsaerf{at}unileon.es.

Journal of Clinical Microbiology, February 2003, p. 880-882, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.880-882.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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