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Journal of Clinical Microbiology, February 2003, p. 886-888, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.886-888.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Transcriptional Analysis of p30 Major Outer Membrane Protein Genes of Ehrlichia canis in Naturally Infected Ticks and Sequence Analysis of p30-10 of E. canis from Diverse Geographic Regions

Suleyman Felek,1 Russell Greene,2 and Yasuko Rikihisa1*

Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio 43210-1093,1 Phoenix Veterinary Internal Medicine Services, Phoenix, Arizona 850282

Received 12 August 2002/ Returned for modification 23 October 2002/ Accepted 22 November 2002

Rhipicephalus sanguineus ticks transmit Ehrlichia canis, the etiologic agent of canine ehrlichiosis. In experimentally infected ticks, only p30-10 transcript was detected among 22 p30 paralogs encoding immunodominant major outer membrane P30 proteins of E. canis. The present study revealed transcription of p30-10 by E. canis in naturally infected ticks and sequence conservation of p30-10 genes for E. canis from diverse geographic regions.

* Corresponding author. Mailing address: Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, 1925 Coffey Rd., Columbus, OH 43210-1093. Phone: (614) 292-5661. Fax: (614) 292-6473. E-mail: rikihisa.1{at}osu.edu.

Journal of Clinical Microbiology, February 2003, p. 886-888, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.886-888.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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