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Journal of Clinical Microbiology, February 2003, p. 889-891, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.889-891.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Direct Identification of Staphylococcus aureus from Positive Blood Culture Bottles

Kenneth Oliveira,1 Stephen M. Brecher,2 Annette Durbin,2 Daniel S. Shapiro,3 Donald R. Schwartz,3 Paola C. De Girolami,4 Joanna Dakos,4 Gary W. Procop,5 Deborah Wilson,5 Chad S. Hanna,6 Gerhard Haase,7 Heidrun Peltroche-Llacsahuanga,7 Kimberle C. Chapin,8 Michael C. Musgnug,8 Michael H. Levi,9 Cynthia Shoemaker,9 and Henrik Stender10*

Applied Biosystems, Bedford,1 Boston VA Healthcare System, West Roxbury,2 Lahey Clinic, Burlington,8 AdvanDx, Concord,10 Boston Medical Center,3 Beth Israel Deaconess Medical Center, Boston, Massachusetts,4 Cleveland Clinic Foundation, Cleveland, Ohio,5 New York University School of Medicine, Bellevue Hospital, New York,6 Montefiore Medical Center, Bronx, New York,9 University Hospital RWTH Aachen, Germany7

Received 15 May 2002/ Returned for modification 26 September 2002/ Accepted 12 November 2002

Fluorescence in situ hybridization (FISH) using peptide nucleic acid (PNA) probes targeting Staphylococcus aureus 16S rRNA is a novel method for direct identification of S. aureus from positive blood culture bottles. The test (S. aureus PNA FISH) is performed on smears made directly from positive blood culture bottles with gram-positive cocci in clusters (GPCC) and provides results within 2.5 h. A blinded comparison of S. aureus PNA FISH with standard identification methods was performed in collaboration with eight clinical microbiology laboratories. A total of 564 routine blood culture bottles positive for GPCC recovered from both aerobic and anaerobic media from three different manufacturers (ESP, BACTEC, and BacT/Alert) were included in the study. The sensitivity and specificity of S. aureus PNA FISH were 100% (57 of 57) and 99.2% (116 of 117), respectively, with 174 GPCC-positive ESP blood culture bottles, 98.5% (67 of 68) and 98.5% (129 of 131), respectively, with 200 GPCC-positive BACTEC blood culture bottles, and 100% (74 of 74) and 99.1% (115 of 116), respectively, with 190 GPCC-positive BacT/Alert blood culture bottles. It is concluded that S. aureus PNA FISH performs well with commonly used continuously monitoring blood culture systems.

* Corresponding author. Mailing address: AdvanDx, 222 Partridge Ln., Concord, MA 01742. Phone: (781) 405-1654. E-mail: Henrik.Stender{at}AdvanDx.com.

Journal of Clinical Microbiology, February 2003, p. 889-891, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.889-891.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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