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Journal of Clinical Microbiology, February 2003, p. 900-904, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.900-904.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.

Identification and Quantification of Ovine Gammaherpesvirus 2 DNA in Fresh and Stored Tissues of Pigs with Symptoms of Porcine Malignant Catarrhal Fever

Sarah Albini,1 Werner Zimmermann,2 Felix Neff,2 Bernhard Ehlers,3 Hansjürg Häni,4 Hong Li,5 Daniela Hüssy,1 Monika Engels,1 and Mathias Ackermann1*

Institute of Virology, Veterinary Medical Faculty, University of Zurich, Zurich,1 Department of Clinical Veterinary Medicine,2 Institute for Animal Pathology, University of Berne, Berne, Switzerland,4 Robert Koch Institute, Berlin, Germany,3 Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington5

Received 8 April 2002/ Returned for modification 6 August 2002/ Accepted 22 November 2002

Cases of porcine malignant catarrhal fever were analyzed by a combination of identification and quantitation of ovine gammaherpesvirus 2 DNA in a variety of paraffin-embedded tissues from diseased pigs, serology, and exclusion of primary porcine gammaherpesviruses. In spite of reduced signal due to fixation and paraffin embedding, ovine gammaherpesvirus 2 DNA in pig brains exceeded the amounts found in sheep brains by orders of magnitude.

* Corresponding author. Mailing address: Institute of Virology, Veterinary Medical Faculty, University of Zurich, Winterthurerstrasse 266a, CH-8057 Zurich, Switzerland. Phone: 41 1 635 87 01. Fax: 41 1 635 89 11. E-mail: ma{at}vetvir.unizh.ch.

Journal of Clinical Microbiology, February 2003, p. 900-904, Vol. 41, No. 2
0095-1137/03/$08.00+0     DOI: 10.1128/JCM.41.2.900-904.2003
Copyright © 2003, American Society for Microbiology. All Rights Reserved.



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Copyright © 2003 by the American Society for Microbiology. All rights reserved.